FastQCFastQC Report
Thu 28 Apr 2016
ERR1041448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11322587
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT406260.35880492682458526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT397150.35075906239448634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT295160.26068247477365375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151540.13383867132131552No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG131980.1165634673418716No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC121250.10708683448402738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147000.022.6278921
GTATCAA230300.014.4031712
ACCGTCG3050.013.3442628
GTACAAA43900.013.0637821
CGTCGTA2850.012.98245610
GTATTAG43150.012.0475091
TAATACT37950.012.0408434
AATACTG26550.011.9849355
TATACTG13550.011.8782285
TTAACGG18750.011.83999935
TCACGTA44700.011.83668925
AATCGTC7050.011.8085128
AAAGGGC43300.011.7494235
CGTTCGG4100.011.73170824
TAACGCC3800.011.6842114
GGACTTA42000.011.67261914
ATCACGT46500.011.53763424
TACAAAG50900.011.5216112
ATTAGAG25000.011.3963
AACGCAG342450.011.3771355
Produced by FastQC (version 0.11.5)