FastQCFastQC Report
Thu 28 Apr 2016
ERR1041447.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041447.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15477680
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1070320.6915248280103995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1047190.6765807278610231No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT795500.5139659173726295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439470.28393790283815146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA354750.018.8467941
AATACTG50600.013.3083015
TTAACGG24750.013.15555635
GTATCAA515650.013.052072
TAATACT60950.012.808864
TCTAATA52500.012.65047552
TATACTG21550.012.619495
ATACTGG46200.012.6136366
TCTATAC19000.012.2684213
GGTAAAC37100.012.26684735
GTATTAG59550.012.1469351
CTCTAAT48450.011.9514961
TAACGGC27300.011.9267436
TAGGACC33950.011.8792344
TACTGGT42450.011.8539467
ACCGTCC5500.011.4363638
GGGGTTA28900.011.2664366
ATTAGAG36100.011.0692533
AACGCAG656800.011.0554975
TAGGGGT25750.010.9203884
Produced by FastQC (version 0.11.5)