FastQCFastQC Report
Thu 28 Apr 2016
ERR1041446.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041446.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7971349
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT245120.307501277387303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT241170.3025460307910242No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT177000.22204522722565528No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG106410.13349057982532192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91150.11434701955716656No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT83300.10449925100506827No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA81760.10256733207892414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93100.021.162731
GTATCAA139200.014.2072562
AATACTG31100.013.97915
TTAACGG18750.013.813333535
GGTAAAC22950.013.70370335
TAGGACC19100.013.560214
TCTAATA31150.013.362762
ATTAGAG23550.013.0403393
ATACTGG29750.012.8722696
TTGGTAA24300.012.86625533
TATTAGA27350.012.8519192
TACTGGT28600.012.6783217
TAATACT40850.012.5899634
GTAAACA25150.012.57852936
GATGCTA26650.012.42589214
TAACGGC20950.012.36276836
TTAGGAC23050.012.0390453
ATGTACG5100.011.97058811
AATCGTC5600.011.89285728
AACGGCC21800.011.88073437
Produced by FastQC (version 0.11.5)