FastQCFastQC Report
Thu 28 Apr 2016
ERR1041445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7984227
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT319850.40060233758383873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT297230.3722714797562745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225670.2826447694936529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150520.18852169408510053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105900.019.5656281
ATCTCGT10500.015.50476337
TTAACGG15250.013.70819735
GTATCAA153050.013.6226722
ATTAGAG20550.013.3236013
CGAGACA12400.013.27822623
TAACGGC16100.013.0993836
CCCACGA12750.013.05882419
ACGAGAC13250.012.845283522
GTATTAG29050.012.6092951
CTACCCG7200.012.59027810
CGAGCCC13150.012.38022815
ATCTCCG13050.012.33333310
TAATACT35550.012.1772154
ACCGCCT19300.012.17357512
AATACTG28250.012.0495585
TATACAC18800.011.8085113
CTAATAC35650.011.572233
TCTCCGA14550.011.57044711
TCTAATA27150.011.4475142
Produced by FastQC (version 0.11.5)