FastQCFastQC Report
Thu 28 Apr 2016
ERR1041444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7823539
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293580.37525217168342867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT281150.35936422123031536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208560.26658012441684004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123200.15747349121669874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89600.021.9068071
GGCGAAT10550.019.6398132
GCGAATC10700.019.01869233
ATCTCGT11250.018.74666837
GACTAAG11700.018.1837626
CCCACGA12450.016.79116419
CGAGCCC13250.016.19622815
CGAATCT12850.015.98054434
ACTAAGG14100.015.35106527
ATCTCCG14250.015.18947410
AGGCGAA13950.014.85304631
TTAACGG15750.014.6825435
ACGAGAC14400.014.64583322
GTATCAA136350.014.4906482
TCTCCGA14750.014.17288111
TATACAC18750.014.1093333
TAACGGC16800.013.87536
AGACTAA15950.013.80250725
GTATTAG33000.013.6787881
GCCCACG15700.013.6687918
Produced by FastQC (version 0.11.5)