FastQCFastQC Report
Thu 28 Apr 2016
ERR1041443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8141095
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT439320.5396325678548155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT422560.5190456566346419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT325300.3995776980860683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201580.24760796919824668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99500.026.5321621
GTATCAA162550.016.3091372
TTAACGG15850.014.1230335
TAACGGC16650.013.66666736
GCGAACA10500.013.39047729
AATACTG30100.012.8455145
ACCGCCT18300.012.838798512
TAATACT36900.012.53387454
GTATTAG32550.012.2764981
AACGGCC18350.012.19891137
TAGGACC18650.012.1018764
ATTAGAG22750.012.0351653
TACTGGT24300.011.9526757
ATACTGG26550.011.9152546
TACCCCG10650.011.8122065
GGCACCG19700.011.7385789
GTACTAT10750.011.7023251
TCTAATA28900.011.5865062
ATCAACG245700.011.5502652
GTGACAC20800.011.47355724
Produced by FastQC (version 0.11.5)