FastQCFastQC Report
Thu 28 Apr 2016
ERR1041442.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041442.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365378
Sequences flagged as poor quality0
Sequence length42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347240.6471864610471061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT278120.5183604957563102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT186130.34690938830404866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74850.139505548350927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60100.025.7271211
GTATCAA142000.020.243661
TTAAGCG2400.015.00000136
TTAACGG4900.013.22448935
TATCAAC217950.013.1562292
ATCAACG217900.013.0518593
TAACGGC4700.013.02127636
AACGCAG219800.012.9636036
TCAACGC219550.012.9619674
CAACGCA222100.012.8050435
TAGACCG1757.407816E-712.3428585
ACGCAGA236950.012.0025337
CGCAGAG237650.011.9747538
AGAGTAC247150.011.485332511
CAGAGTA248850.011.42133810
GTATTAG15400.011.3376631
GCAGAGT250700.011.3370579
TTAGACT7500.011.044
GTATAAA15150.010.8118821
GTAGCAC5500.010.83
Produced by FastQC (version 0.11.5)