FastQCFastQC Report
Thu 28 Apr 2016
ERR1041441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9889335
Sequences flagged as poor quality0
Sequence length42
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT645330.6525514607402823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT477840.4831871910497521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT310330.31380269755246437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126740.12815826342216136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102200.025.3091981
GTATCAA255450.021.1391661
TATCAAC386450.013.8755342
ATCAACG384850.013.8583863
AACGCAG387750.013.7222446
TCAACGC389550.013.6865614
CAACGCA392950.013.5681385
GTATTAG32600.013.5276081
ACGCAGA417100.012.7523377
CGCAGAG421100.012.6397538
TTAACGG10700.012.44859835
AGAGTAC439900.012.00545611
TATACTG27700.011.9566795
CAGAGTA443450.011.92964310
TATACCG4100.011.8536595
GCAGAGT446650.011.8482049
TAACGGC11100.011.83783836
CGAACTA10400.010.90384629
CCGTTAA9350.010.78074916
GCGAACT10600.010.52830228
Produced by FastQC (version 0.11.5)