FastQCFastQC Report
Thu 28 Apr 2016
ERR1041440.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041440.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746222
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26080.14935099889933812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.1179116973672305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18780.10754646316447737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC601.3380723E-624.6666663
GGTATCA9550.024.4083771
TCTAACG851.246066E-619.5882362
GTACTAT1900.018.51
TCTATAC2850.017.5263163
CCGTTTA752.0680415E-417.26666613
TAACGCC2050.017.1463414
GTATACT1950.017.0769234
GTAATCG1851.8189894E-1217.00000220
CGTATTA1107.813305E-716.81818215
GGGGTTA3350.016.5671636
ACCGCGT904.4480847E-516.4444458
GTACTAG1352.2213499E-816.4444431
ATCGATA2050.016.24390223
GCGTTTA2400.016.187532
TCTAGCG1951.8189894E-1216.12820628
TAATCGA1951.8189894E-1216.12820621
ATACCAT2550.015.9607846
AATCGAT2100.015.85714422
CTATACC4450.015.3820224
Produced by FastQC (version 0.11.5)