FastQCFastQC Report
Thu 28 Apr 2016
ERR1041439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927633
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142470.7390929704980149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132770.6887721884819361No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95460.4952187475520496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66820.3466427478674623No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24730.12829205559356993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG957.1522663E-921.42105321
TATACCG1303.274181E-1119.9230775
TACCGAC1301.3948011E-817.0769237
GTCGTCA1201.04224455E-716.95833224
GTACTAG2200.015.9772731
TATACTG4550.015.8571435
ATCTACG700.002593348315.8571421
TCGTATT2350.015.74467926
CTAGTAC2250.015.6222223
GCATTCG2150.015.48837222
ATACCGA1802.0190782E-1015.4166666
GGTCTAT1252.9610765E-614.8000011
TGTTCGG1001.0939741E-414.79999910
GGTATCA66850.014.556471
GTATACT2950.014.4237294
CGGGATA908.2775985E-414.38888824
CTGTGCG1803.3342076E-914.3888889
CGTATTG2353.6379788E-1214.17021227
GACGGCC2750.014.12727411
ATAATAC3550.014.0704223
Produced by FastQC (version 0.11.5)