FastQCFastQC Report
Thu 28 Apr 2016
ERR1041438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187328
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29861.5939955585924155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23241.2406047147249744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.9427314656645029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8470.4521481038606081No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5060.27011445165698666No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.25303211479330373No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.22847625555175946No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.19537922787837375No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3560.19004099760847284No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.16922189955585926No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2630.14039545609839427No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2610.13932781004441408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2180.1163734198838401No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.10196019815510762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA200.001838005837.01
TCGTAAG200.001838005837.02
GCGTCAG303.5880867E-430.8333322
TTACCAG250.00548474929.610
TAGTGTA358.841589E-426.42857231
CGAAAGC809.658834E-1025.437519
GCGAAAG809.658834E-1025.437518
GCGCAAG809.658834E-1025.43751
ACGGACC809.658834E-1025.43758
CGGACCA809.658834E-1025.43759
GACGGAC901.4006218E-1024.6666667
AGCGAAA851.9499566E-923.94117717
CGCAAGA851.9499566E-923.9411772
CAGAGCG851.9499566E-923.94117714
AAGACGG952.764864E-1023.3684225
CAAGACG952.764864E-1023.3684224
GGGTAAA400.001925190723.1251
CCGTAGA400.001925190723.1252
GTATACT400.001925190723.1254
GAGCGAA903.776222E-922.6111116
Produced by FastQC (version 0.11.5)