FastQCFastQC Report
Thu 28 Apr 2016
ERR1041437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618772
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT186621.1528491967985608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160970.9943957518415194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124020.7661363057922919No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85290.52688087019049No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA33060.20422888461129793No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30320.187302473726998No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28830.17809796561838231No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.17636825939662906No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA24080.14875473507078205No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.13269317729735874No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.13145767285324927No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA17820.11008344597015515No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG16820.10390592374960772No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16710.10322639630534751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTA555.1443407E-420.18181831
TAGGTCG1306.9667294E-1018.521
CATTGCG1351.1532393E-917.81481629
ACGGACC2350.017.3191498
TACGACT650.001580310517.0769234
AAAGCCG2100.016.73809415
CGCCAAT1452.9813236E-916.58620614
CTGTGCG2150.016.3488379
GTCGTCA1606.2937033E-1016.187524
TGTGCGC1951.8189894E-1216.12820610
TGCGACG1151.2428864E-616.08695622
TATACCG1151.2428864E-616.0869565
TAGACTG1504.6748028E-916.0333335
CGTCATT1504.6748028E-916.03333326
CGAACGT1059.347737E-615.8571424
TATCTAG2450.015.8571421
ATACCGA1059.347737E-615.8571426
TACCGAC700.002593107515.8571427
CTAGTAC2003.6379788E-1215.7253
TATACTG4500.015.6222215
Produced by FastQC (version 0.11.5)