FastQCFastQC Report
Thu 28 Apr 2016
ERR1041436.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041436.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319585
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT204001.5459405798034989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177021.341482359984389No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133321.010317637742169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88620.6715747754028728No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA36200.2743286715141503No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32510.24636533455593992No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA28100.21294573672783487No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG27900.2114301087084197No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26670.20210899638901625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23030.1745245664356597No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.16391517029975333No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA19960.15125967633763646No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG17900.13564870773765994No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.12867681884835006No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA16740.12685806522505183No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA13810.10465411474061921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTT450.003826103420.5555577
TTGTGCG500.007035420318.522
CGATCGT500.007035420318.513
ACGGACC2400.017.7291688
CTAGCGG1156.4070264E-817.69565229
GCGCAAG2200.017.659091
GACGGAC2200.017.659097
CGAGCCG2100.017.6190515
ATACCGA852.72361E-517.4117646
TACGCTG650.001580101417.0769235
AAGACGG2400.016.9583345
CAATACG1452.9795046E-916.5862064
CGCAAGA2350.016.5319142
GCGTGCT803.3830397E-416.18759
ACCTCGT700.00259276615.85714315
ACGTTCG957.06094E-515.5789487
CGTCGTA1557.210474E-915.51612910
TAGACTG1802.0190782E-1015.4166675
TACCGTC1802.0190782E-1015.4166677
CTGTGCG1455.3467375E-815.3103459
Produced by FastQC (version 0.11.5)