FastQCFastQC Report
Thu 28 Apr 2016
ERR1041435.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041435.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894233
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163930.42095580824259876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153070.39306841681019083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118990.30555439286760705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.18375890708131742No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC45390.11655697026859975No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG41700.10708142014101364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA650.001580849917.07692314
TATATCG1001.09442946E-414.7999995
TACCGTC4900.014.724497
TTAGACT4400.014.2954544
CGTCGTA4350.014.03448210
CTAGTAC3050.013.950823
GTATTAG8650.013.68786051
TATACTG8450.013.5739655
TCTAGCG3850.013.45454528
CTAGCGG4150.013.37349429
ATACCGT6500.013.0923076
CGCAATA4400.013.03409136
ACCGTCG4900.012.8367368
TAACGCC5200.012.8076934
CAAGACG8100.012.7901234
TAGTACT6000.012.6416664
CCGTCGT4900.012.4591859
AAGACGG8200.012.4085375
ACAGCGT3750.012.3333348
TAGGTCG1501.9462404E-512.33333321
Produced by FastQC (version 0.11.5)