FastQCFastQC Report
Thu 28 Apr 2016
ERR1041434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442659
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107390.7443893532705927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102250.7087606981275547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53500.37084300586625113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45890.3180931876486404No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14670.1016872317020169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCG2500.017.7633
CTATAGT1352.2204404E-816.4444454
GACGGAC3150.016.4444457
TCTACAC1708.54925E-1116.323533
CGGCGAT2850.016.2280730
ACGGACC3200.016.18758
TAGACTA1557.212293E-915.5161295
TGGTCGG2055.456968E-1215.34146437
GCGATGC2900.015.31034532
CGTATAC855.365328E-415.2352943
CGTCGTA1701.4861143E-915.23529410
CAGTCCG855.365328E-415.2352949
TACCGTC1954.1836756E-1115.1794877
CCTATAC1853.0559022E-1015.03
TCCGACC3000.014.88
GTACTAG1001.0936707E-414.81
GTATACT2006.184564E-1114.84
ACCGTCG1752.239176E-914.88
AAGACGG3750.014.7999995
CGCAAGA3400.014.6911762
Produced by FastQC (version 0.11.5)