FastQCFastQC Report
Thu 28 Apr 2016
ERR1041433.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041433.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822379
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75710.9206217571217163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56500.6870311620311317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34610.4208521861574773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19500.23711694972755873No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT11380.1383790198922881No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.10518264693042988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG556.2581057E-726.909092
GTCGCGT1450.022.9655178
TCGCGTA1500.020.9666679
CGCGTAA1550.020.29032110
GTATACG555.1413826E-420.1818181
ACGAACG759.260511E-619.73333415
CCGATAA851.244427E-619.5882369
GGTCGCG1650.019.0606067
CAAGACG2850.018.8245624
AAGACGG2700.018.55
TTCCGAT801.6157588E-518.57
GTAATAC1103.8477083E-818.53
TCTAGAC500.007033223318.4999983
CCCGTAG500.007033223318.4999981
GCGTAAC2000.018.49999811
CGTAACT2100.017.61904712
TATACTG2100.017.6190475
AGACGGA2750.017.490916
GCGCAAG2650.017.452831
CGAACGA852.7215901E-517.41176416
Produced by FastQC (version 0.11.5)