FastQCFastQC Report
Thu 28 Apr 2016
ERR1041432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348874
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106090.3167930474541592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.2670748436638703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73350.21902884372478632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.13288048460467608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38700.020.1253221
ACGGACC4300.016.3488378
GACGGAC4350.016.160927
TAATACG1902.7284841E-1115.5789484
AGACGGA5200.014.2307696
AAGACGG6250.014.2085
TTAACGG1702.4414476E-814.14705935
GTATCAA55700.013.8833042
ATAACGC4050.013.7037043
TCTAGCG2304.0017767E-1113.67391328
TATACTG9200.013.6739135
TAACGCC3250.013.6615384
TAACGGC1907.1395334E-913.63157936
GTATTAG8200.013.5365851
TTAGCGC1102.4585627E-413.4545453
GCGAAAG5450.013.23853218
CGGACCA5650.013.0973459
CGCAAGA5800.013.0775862
ACCGTCG2551.6370905E-1113.0588248
TATACCG1857.3967385E-813.05
Produced by FastQC (version 0.11.5)