FastQCFastQC Report
Thu 28 Apr 2016
ERR1041431.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041431.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899898
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148500.5120869768522893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130260.44918821282679594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105940.36532319412613823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.21786973196988307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2450.018.8775529
GCGTTAG500.007037405418.4999981
TAACGCC2950.018.1864414
TGCGACG1301.3955287E-817.07692322
TATACCG1107.817689E-716.8181825
CGTCGTA2450.016.61224410
ACCGTCG2500.016.2799998
TACCGTC2850.016.228077
TTACGGT803.384788E-416.18754
CGCAATA3450.016.08695636
CCGTCGT2700.015.7592599
TATACTG5900.015.6779665
TCTAGCG2850.015.57894728
AACGCCG3150.015.2698415
GGTATCA66800.014.8720051
AAGACGG5200.014.5865385
TACCCCG1406.003429E-714.5357155
TAGCGGC3350.014.35820930
TACGACG3350.014.3582095
ACGGACC4650.014.322588
Produced by FastQC (version 0.11.5)