FastQCFastQC Report
Thu 28 Apr 2016
ERR1041430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4601638
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT168140.36539162793770397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143310.31143258118087513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121310.2636235184080104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76910.16713613717550144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.017.5495151
GACGGAC6450.016.3488377
ACGGACC6550.016.0992368
TATACTG13550.015.1549815
AAGACGG8200.015.1158535
GTCGCTA1304.4506633E-614.23076937
CGGGTCG2101.364242E-1014.09523834
CGTATAC1356.5767654E-613.7037043
CTAACGC950.001246068313.6315793
GTACTAG3000.013.5666661
GTATTAG10950.013.5159831
TAACACG3750.013.324
CGGACCA8200.013.0853659
CGCAAGA9400.012.9893622
GCGCAAG10400.012.9855771
CTAATCG2001.4659236E-812.95000126
TCTAGCG3600.012.84722228
TCTACAC7800.012.8076933
CGACGGT4350.012.7586217
ACGAACG3850.012.49350615
Produced by FastQC (version 0.11.5)