FastQCFastQC Report
Thu 28 Apr 2016
ERR1041429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206414
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169750.32604014970764905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT145910.28025047566328765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121100.2325977150491682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68250.1310883076144156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.020.046721
CGAACGA3300.015.13636416
TATACTG12500.014.9479995
ATACCGT7400.014.56
TACCGTC5850.014.2307687
ATAACGC6900.013.9420283
TCGTTTA5600.013.87500130
TAACGCC6300.013.8015874
CGCATCG5900.013.79660913
GTATCAA89800.013.5762822
TAGTACT7850.013.4331214
ACGAACG3650.013.17808215
CCGTCGT5500.013.1181819
ACCGTCG5500.013.1181818
CGTCGTA5100.013.05882410
CTAGCGG4550.013.01098829
CCGGTCG6200.012.83064620
TAATACT14750.012.6677974
CGCGCTA6950.012.51079124
ACGTAAG1807.4772106E-712.3333331
Produced by FastQC (version 0.11.5)