FastQCFastQC Report
Thu 28 Apr 2016
ERR1041427.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041427.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8174409
Sequences flagged as poor quality0
Sequence length42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT557440.6819330914320534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT435210.5324054619728472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT264060.32303252748914324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119240.14586987267214058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88000.026.7545451
GTATCAA230700.021.3472041
TTAACGG12900.016.32558335
TAACGGC13000.016.19999936
TATCAAC344900.014.1954182
ATCAACG346150.014.05575753
TTTAACG16050.014.01869334
TCAACGC350750.013.8560234
AACGCAG350550.013.8536596
CAACGCA354400.013.7133195
GTATTAG33250.013.1007531
CGCGACT1807.401832E-813.017
ACGCAGA377000.012.8769237
CGCAGAG382700.012.6945398
CGCGTAA1000.002253556412.59999910
GCGAACT11300.012.26548728
CAGAGTA398800.012.15045210
AGAGTAC399550.012.11863311
GCAGAGT400550.012.0704039
TACCCCG9150.012.0000015
Produced by FastQC (version 0.11.5)