FastQCFastQC Report
Thu 28 Apr 2016
ERR1041426.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041426.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8340607
Sequences flagged as poor quality0
Sequence length42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT461540.5533650008926209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346680.4156532012598124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT217330.26056856533343437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92120.11044759691950477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71350.027.8766631
GTATCAA190600.021.4375671
ATCAACG283350.014.1916373
TCAACGC284400.014.1012664
GTATTAG38100.013.9842521
TATCAAC289600.013.94129852
CAACGCA288100.013.938915
AACGCAG292050.013.7996926
GCGAACT15350.013.60260628
TTAACGG18650.013.31903535
CGAACTA15450.013.28155429
TAACGGC18700.013.18716636
ACGCAGA312450.012.8929437
TAGGACC13600.012.8382364
CGCAGAG316250.012.7380248
CAGAGTA330050.012.17815510
AGAGTAC329550.012.16932211
GCAGAGT330250.012.15442859
GTATACG4000.012.151
GTATTAA18400.012.0326091
Produced by FastQC (version 0.11.5)