FastQCFastQC Report
Thu 28 Apr 2016
ERR1041425.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041425.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8577851
Sequences flagged as poor quality0
Sequence length42
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT553810.6456279084353412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT406160.47349854876238817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT262430.30593909826598764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114510.13349497444056793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88850.024.067531
GTATCAA228100.021.5668561
TAACGGC18100.015.11602236
TTAACGG18500.014.88648735
TATCAAC344500.014.20667652
ATCAACG348650.013.9652953
AACGCAG348300.013.9638246
TCAACGC351150.013.8504924
CAACGCA355500.013.6759495
GTATTAG36650.013.4079131
ACGCAGA371750.013.0829857
CGCAGAG375000.012.96968
CCGTTAA16850.012.81899116
AGGTCGC19100.012.81675435
AGAGTAC391200.012.37269911
TTTAACG23050.012.33839534
CAGAGTA392800.012.33146610
GCAGAGT395950.012.2242719
TAGGACC17550.012.2051294
GCCGTTA17750.012.06760515
Produced by FastQC (version 0.11.5)