FastQCFastQC Report
Thu 28 Apr 2016
ERR1041424.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041424.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19285247
Sequences flagged as poor quality0
Sequence length42
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCG1712130.88779262199753No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT647810.335909620447174No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGC522010.2706784102894819No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT490910.254552093628876No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT363410.18843938063121515No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT206850.10725815438091096No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACC193450.10030983787762739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA231650.030.9648192
CTTATAC233950.030.7681121
TATACAC235450.030.5415153
GGTATCA126850.025.6980691
GCCGTCT80350.024.05973836
TACACAT330300.022.141695
ATACACA334000.021.8317364
TGCCGTC92100.021.22475435
ACACATC411350.017.958436
ACATCTC439050.016.7352248
CACATCT444950.016.379827
GTATCAA199400.016.2577722
ATGCCGT123350.015.94973734
ATCTCCG472450.015.319715510
CCGTCTT26900.014.65427536
CATCTCC527050.013.8521969
CCTGTAA187400.013.706511
GTATGCC155750.013.61412532
TATACTG17700.013.4237295
TCTCCGA541300.013.34121511
Produced by FastQC (version 0.11.5)