FastQCFastQC Report
Thu 28 Apr 2016
ERR1041423.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041423.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742674
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT294670.3805791125908181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269460.3480193018587635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198780.25673300981030583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.027.036021
GTATCAA138200.017.0408822
GTATTAG15150.012.4554461
AACGCAG211150.011.7842765
ATCAACG208900.011.7429392
TATACTG10250.011.7317085
TCAACGC211300.011.7058693
CAACGCA213900.011.5895284
TATCAAC214550.011.2181311
TTAACGG4500.011.135
ACGCAGA234300.010.6357246
CGCAGAG236900.010.5346137
TAAACGC5300.010.47169828
TAATACT16800.010.4613094
CGTATAC2301.2445435E-610.45652224
CTACACT17750.010.3183114
CTAGTAC4201.8189894E-1210.1309523
TCTACAC16100.010.1118013
CGGTCGA2208.318997E-610.09090920
AGAGTAC248100.010.07396210
Produced by FastQC (version 0.11.5)