FastQCFastQC Report
Thu 28 Apr 2016
ERR1041422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7543991
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT277910.36838591138298016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT257050.3407347649274767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT201350.2669011667696846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78500.028.6808911
GTATCAA131150.017.1951962
TATACTG8800.011.7727275
ATCAACG198700.011.3402122
TATCAAC203150.011.31946853
TCAACGC200650.011.2668833
TTAACGG4600.011.2608735
TAATACT16150.011.22600654
CAACGCA203350.011.1536764
AACGCAG205200.011.0801665
TAACGGC4850.011.06185536
TCTATAC8950.010.7486043
GTATAGG8800.010.3011361
TTAGACT8850.010.2429384
GTATTAG18000.010.0722231
TAGTACT9000.010.0722234
AGGACCG7550.010.0463585
TTGCGCG12800.09.97265618
ACGCAGA228600.09.9378826
CGATCGC9750.09.86666719
Produced by FastQC (version 0.11.5)