FastQCFastQC Report
Thu 28 Apr 2016
ERR1041421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9573539
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT849960.88782215228872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT841550.8790375220699472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT592210.6185904710891134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230140.24039177152774954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162950.032.072721
GTATCAA270850.019.3230572
GACCGTA7950.015.12578726
ACCGTAC8250.014.79999927
CGTACTA8200.014.66463429
ATCAACG441900.013.1664412
TCAACGC450700.012.966833
ATCTCGT9600.012.91145837
TATCAAC444750.012.8699261
CAACGCA457800.012.7859334
AACGCAG471150.012.4707635
TATACTG14350.012.2473875
GTCGGGA23850.011.9454932
CCCACGA10500.011.62857119
ATCTCCG11150.011.61434910
GTACTAG11050.011.21719430
ACGCAGA528600.011.1678966
TATACAC17950.011.1309193
CGCAGAG534850.011.0581477
GGTCGGG27750.010.5333341
Produced by FastQC (version 0.11.5)