FastQCFastQC Report
Thu 28 Apr 2016
ERR1041420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13177847
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT639560.4853296596932716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT617000.46821001943640717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT455710.34581521549005695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207930.15778753539937138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248550.018.0496881
GTATCAA355350.012.60405352
TTAACGG8900.012.26404535
TATACTG18350.011.8964585
GTATTAG38450.011.5955791
CTAGTAC9150.011.52459053
GTATAGG18850.011.3846161
TCTATAC16900.011.3846153
AACGCAG455250.011.09390455
TAACGGC10100.010.99009836
TAATACT34750.010.7539584
GTCTATA10100.010.6237621
CGATCGC18000.010.58611119
CTAGATA19800.010.5580813
ATAATAC24400.010.5389353
TATTAGA25100.010.245022
ATCAACG496150.010.0861632
TATCTAG25100.010.0239041
TCAACGC501450.010.0164523
CAACGCA504000.09.998814
Produced by FastQC (version 0.11.5)