FastQCFastQC Report
Thu 28 Apr 2016
ERR1041419.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041419.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4839189
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT298280.6163842743071204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT282610.5840028153477783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188980.3905199817572738No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79190.16364312284558424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76850.029.5133381
GTATCAA121950.018.5834352
ATCTCGT5150.015.08737937
CCCACGA6100.013.04098319
TATACAC8300.012.704823
TCTATAC4850.012.5876293
CGAGCCC6700.012.42537315
ATCAACG191350.012.2205382
CTAGACT5800.012.120694
GTCGGGA9700.012.0154642
TCAACGC195600.011.9833843
ATCTCCG6200.011.93548410
CAACGCA197900.011.8721584
AACGCAG199200.011.8689775
TATCAAC196400.011.7367623
GCTATAC4900.011.326533
CCGAGCC9150.011.12021914
CGAACGT7850.011.0764334
TAATACG2851.4224497E-911.0350884
TCGAACG8250.010.7636363
Produced by FastQC (version 0.11.5)