FastQCFastQC Report
Thu 28 Apr 2016
ERR1041418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5558624
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT370530.6665858313136488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365150.656907177027984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257100.462524538446925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115400.2076053354211402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118350.021.2120821
GTATCAA179700.013.9908192
CGAACGC2009.858923E-1013.87499930
ATACGAC850.00941066613.0588243
TAGGTCG1451.9407617E-411.48275855
GTATTAG15500.011.4580641
CGACTAA1951.9639756E-611.38461637
AACGCAG242700.011.2128135
CGAATCG1502.6582938E-411.116
ACGAACG2553.641071E-810.88235329
TATACTG8950.010.7486035
TAATACT15750.010.5714284
ATCAACG257200.010.5231342
ATACCGA2656.4052074E-810.4716986
TCAACGC262250.010.3557673
TACACTG13600.010.3382365
TAATACG3606.002665E-1110.2777774
AATACTG11350.010.2687235
CTAGTAC3251.1350494E-910.2461533
TATCAAC260250.010.243421
Produced by FastQC (version 0.11.5)