FastQCFastQC Report
Thu 28 Apr 2016
ERR1041417.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041417.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6849883
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT579930.8466275993327185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT563500.8226417881882071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT401850.5866523559599485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180200.2630701867462554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135600.026.631271
ATCTCGT9600.020.23437537
CCCACGA10750.017.89767519
ACGAGAC11500.017.53478222
GTATCAA215800.016.7597312
TATACAC15100.016.049673
ATCTCCG12600.016.0039710
CGAGCCC12850.015.4046715
TCCGAGC13200.015.13636413
TCTCCGA13600.014.96323611
CGAGACT14000.014.53571523
CACGAGA14000.014.40357221
GCCCACG14500.014.28965518
GATCTCG15150.013.18811936
CCGAGCC16450.013.04559314
ACTAGGC15450.012.93203827
CCACGAG15600.012.80769220
GTCGGGA13250.012.705662
CTTATAC17050.012.6950151
ATCAACG334950.012.4217052
Produced by FastQC (version 0.11.5)