FastQCFastQC Report
Thu 28 Apr 2016
ERR1041416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7907728
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT797101.0080012868424406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT761460.962931451359986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT532370.6732275060548365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246600.3118468414695093No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT82330.10411334330164113No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT81800.10344311286377074No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.10076219111228915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200350.024.1280251
CGAGACG13050.023.39080423
ATCTCGT13900.021.82733737
TATACAC17550.020.028493
GACGGAC15350.019.76547226
ACGAGAC15800.019.55379722
CCCACGA16100.018.8447219
ATCTCCG16850.018.44510510
TATCTCG17000.018.06470736
TCTCCGA18900.016.73809611
AGACGGA18650.016.56568325
CGAGCCC18650.016.46648815
CGGACTC18950.016.1081828
TTATACA20850.016.0599522
TCCGAGC19800.015.79040413
GTATCAA307350.015.73417952
GAGACGG20400.015.05392224
CACGAGA20300.015.03694421
TACACAT24500.014.951025
CCGAGCC23300.014.7682414
Produced by FastQC (version 0.11.5)