FastQCFastQC Report
Thu 28 Apr 2016
ERR1041415.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041415.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8527930
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT490360.575004719785458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399650.4686365859006817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT264540.3102042347908578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150190.17611542308625894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6000.014.4916671
ACGAGAC10100.014.28712922
GGTATCA204550.014.1271091
ATCTCGT11050.013.56108537
TATACAC16650.013.4444453
CACGAGA11250.012.82666621
ATCTCCG11650.012.54506510
CCCACGA11800.012.54237319
CGAGACT12950.012.42857223
TAGACTG13200.012.053035
AACGCAG307700.011.9826135
ATTCGCG1401.4003259E-411.89285730
TAGTACT9300.011.7365594
CTACACT14400.011.3055554
TCTCCGA13750.011.30181911
CTAGTAC7100.011.2042263
GAGACTA14300.010.99650424
CCTACAC16350.010.9755343
CGCAGAG338600.010.8891027
TATACTG14300.010.8671335
Produced by FastQC (version 0.11.5)