FastQCFastQC Report
Thu 28 Apr 2016
ERR1041414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5547967
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT262170.4725514769644448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204270.36818892397881964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT108380.1953508375230062No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG97370.1755057302972422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92880.16741267567020496No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT80960.14592732797437333No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG69900.1259920976458584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT15050.024.21594637
GACGGAC15400.023.90584626
CGAGACG15400.023.90584623
ACGAGAC15600.023.71794922
TATACAC16450.023.617023
TATCTCG16000.023.12499836
ACGGACT16100.022.98136727
ATCTCCG16550.022.24471310
CCCACGA17850.020.83193219
CACGAGA18000.020.65833321
CGGACTC18100.020.544228
TCCGAGC18250.020.47671313
CTTATAC18600.020.190861
TCTCCGA18950.019.62269211
GAGACGG19050.019.51968424
AGACGGA19250.019.5090925
CGAGCCC19050.019.42257115
TCCTATC19600.019.34949133
ACTCCTA19500.019.16410431
ATACACA22650.018.5408384
Produced by FastQC (version 0.11.5)