FastQCFastQC Report
Thu 28 Apr 2016
ERR1041413.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041413.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8948672
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT333640.3728374444833826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT315920.3530356236098496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224020.250338821223976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98740.11034039464179714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98000.020.4443871
GTATCAA153050.013.1029082
AACGCAG206250.012.4140615
ATCAACG214800.011.7476722
CAACGCA219400.011.5941214
TCAACGC219150.011.5735793
GTATAGG11150.011.4484311
TATACTG13050.011.3409965
GTATTAG18950.011.3245381
TAGACTG14800.011.2500015
ACGCAGA230050.011.1860466
CGCAGAG233150.011.045257
TATCAAC226350.011.017451
CTAGTAC7100.010.9436623
AGAGTAC241900.010.58453810
CAGAGTA246900.010.5200499
TCTATAC13950.010.4767033
GCAGAGT252450.010.2594588
TACACTG20150.010.1910675
GTCTTAC9100.010.1648351
Produced by FastQC (version 0.11.5)