FastQCFastQC Report
Thu 28 Apr 2016
ERR1041412.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041412.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6603904
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT508980.7707259221212179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT456240.6908640707072665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT348970.5284298499796485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151890.23000031496520848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174650.018.4099641
GTATCAA252450.012.7070712
TCTATAC9300.012.3333333
TTTAGCG4400.012.19318226
AACGCAG325850.011.7125985
TACTCGC3401.8189894E-1211.42647120
CTAACGC1300.001004025611.3846153
CTAGTAC4300.011.1860473
ATCGATA5150.011.13592223
TATACTG9700.010.8711345
CTACACT14600.010.7705484
GTATAGG8850.010.6610171
ATCAACG356700.010.6321842
ACGCAGA360450.010.6139556
ATCTCGT6500.010.53076937
CGCAGAG364300.010.5271767
TCAACGC362150.010.50283053
CAACGCA363950.010.455974
CGTATAC3554.5474735E-1110.4225353
TATCAAC361050.010.406731
Produced by FastQC (version 0.11.5)