FastQCFastQC Report
Thu 28 Apr 2016
ERR1041411.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041411.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12015105
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT576380.4797128281442401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT566830.4717644997692488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT401660.3342958717381163No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186550.15526289616278843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182600.021.8636381
GTATCAA275650.014.4496642
AACGCAG386100.011.74397855
ATCAACG399400.011.2046822
TCAACGC404600.011.083543
TTAACGG6550.011.01526735
CAACGCA408350.011.013474
GACCGTA5950.010.88235326
CTACACT24450.010.8200414
TATCAAC409600.010.8037121
ACGCAGA426650.010.6277986
CGCAGAG432100.010.4809077
GTATTAG31300.010.3434511
AGAGTAC453650.010.00341710
TCTATAC14950.09.8996673
ATAACGC5800.09.8879313
CAGAGTA462350.09.8832059
TACACTG28000.09.8446435
GCAGAGT465900.09.84363658
TATACTG18150.09.7851245
Produced by FastQC (version 0.11.5)