FastQCFastQC Report
Thu 28 Apr 2016
ERR1041410.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041410.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9414506
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT240930.25591358696887545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT226660.24075612676862707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT155480.1651493981734145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102300.022.0263921
GTATCAA156850.014.38954452
GTCTAAC6250.011.841
AACGCAG223000.011.7221985
TCTATAC10450.011.6842123
ATCAACG226050.011.351252
GCGAATC6200.011.3387133
CAACGCA230250.011.2486434
TCAACGC231550.011.17753
CTAGATA13850.010.9530683
GTATTAG29950.010.9332221
GTATAGA12200.010.9180331
GTACTAG5150.010.7766981
TAGGACC16350.010.7492354
ACGCAGA244850.010.6761286
TATCAAC238750.010.6699491
CCTACAC19750.010.584813
TACACTG18200.010.4697815
CGCAGAG251800.010.4328837
CGCGGTC10650.010.42253632
Produced by FastQC (version 0.11.5)