FastQCFastQC Report
Thu 28 Apr 2016
ERR1041409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4788791
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT172260.35971500948778096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT155050.32377691989481266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107470.22441990055527583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50930.10635252196222386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77100.018.212061
TATACTG6050.013.760335
GCGTAAT850.009410419513.0588231
CTAGTAC2900.012.7586193
GTATCAA113050.012.3878812
GTGTAGG8600.011.4011621
GTACTAG2603.983587E-911.3846151
TAGTACC3750.011.3466664
AACGCAG148300.011.3395155
GTATACG2301.0253643E-711.260871
ACACCGT4000.011.16
TACCGTC2553.6405254E-810.8823537
CGAACGA1400.001863759510.57142916
TATATCG2104.7751073E-610.5714285
TACGTAG2104.7751073E-610.57142814
ACGCAGA162500.010.43969256
ATCAACG160000.010.406252
TCAACGC163300.010.2865893
CAACGCA163600.010.245114
TCTAACG1450.002494966610.2068962
Produced by FastQC (version 0.11.5)