FastQCFastQC Report
Thu 28 Apr 2016
ERR1041408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3857107
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152850.39628146172766276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT149780.3883221284760833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90530.23470958933729347No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45370.11762701942155092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35300.027.1997171
GTATCAA55500.017.22
TACACTG7900.012.8797465
ACGAACG1153.5805715E-412.86956529
TATATCG1602.6994821E-612.718755
TAGGTCG1050.002645516812.3333335
ATCAACG82900.012.072982
TCAACGC84050.011.8637723
CAACGCA85050.011.8330394
AACGCAG87200.011.5412855
ATAAGAC4350.011.4827583
TATCAAC85400.011.4812651
TATAGAC3600.011.3055563
CGGTTAA1150.0052222511.2608731
CTAGACT5600.010.9017854
TAAACGT1706.987904E-510.8823534
TATACAG5450.010.8623855
CGTAAAC2259.3768176E-710.6888893
GACCGTG4200.010.5714287
CTAATCG1400.001863618410.57142826
Produced by FastQC (version 0.11.5)