FastQCFastQC Report
Thu 28 Apr 2016
ERR1041407.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041407.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383241
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT184990.7762118896074716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT167220.7016495604095431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83220.3491883531711648No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64100.26896146885690536No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.10653559585455269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.029.5724931
GTATCAA52650.022.5935422
TACGAAC500.00703704518.528
GCGTATA904.4491808E-516.4444452
TAGCCCG1151.2434884E-616.0869565
TATCAAC82900.014.2599523
ATCAACG83550.014.2597254
TACCGTC1051.6568077E-414.0952397
TCAACGC85150.014.0786855
CAACGCA85550.013.9696086
TTACACG800.0063012413.8754
CGTAGCG950.001245759913.63157915
AACGCAG89600.013.3175237
TTAGACG850.009408602513.0588243
GTATACG1000.001833484212.9500011
CGCTAAG1153.5796777E-412.8695651
ACTATCG1451.3733259E-512.758628
GTAAGAC2204.090907E-912.6136363
TATACAC4400.012.6136363
CGTATCG1050.00264500412.33333415
Produced by FastQC (version 0.11.5)