FastQCFastQC Report
Thu 28 Apr 2016
ERR1041406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4886841
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT218930.4479990243185731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT206960.42350467305975376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137760.28189990220676303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69250.14170708643886717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72800.020.4059091
GACGGAC4400.015.97727326
ATCTCGT4600.014.07608737
GTATCAA107900.013.6478222
CGAGACG5350.013.48598123
TATACAC8650.012.832373
TATACTG7550.012.7417215
AACGCAG140650.012.7060075
GTATACG2451.5661499E-912.0816331
ACGAGAC6050.011.9256222
TAGCGCG1257.2260847E-411.8429
TATCTCG5950.011.81512536
ATCAACG152550.011.6057022
CGTATCG1603.768516E-511.56250113
CCTATGC3850.011.5324693
GTACCGT1451.9406696E-411.4827596
TCAACGC155850.011.4786653
CAACGCA155800.011.4348534
ACGCAGA158850.011.2618826
CGCAGAG162750.011.0488477
Produced by FastQC (version 0.11.5)