FastQCFastQC Report
Thu 28 Apr 2016
ERR1041405.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041405.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5453345
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT657661.205975415089271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT508600.9326385915433555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT354580.650206432932448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228130.4183304008823942No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74710.13699848441644533No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70960.13012197101045322No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.10113059049079053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC9550.020.5340322
ATCTCGT10150.019.13793237
CCCACGA10750.018.93023319
CGAGACA10300.018.85922223
AATCTCG11050.018.08144836
ATCTCCG12150.016.74897210
TCTCCGA11850.016.70464111
CACGAGA12150.016.13991721
TATACAC14600.015.9657533
TCCGAGC13200.015.13636513
CGAGCCC13550.014.60885615
GCCCACG14200.014.20070418
CCGAGCC15150.013.67656814
CCACGAG14650.013.38566520
TACACAT18850.013.3474815
GGTATCA286100.013.26232051
CGATCGT1153.5809944E-412.86956526
GTATTAG13200.012.7537891
AACGCAG358800.012.6066055
CTCCGAG17050.012.47800612
Produced by FastQC (version 0.11.5)