FastQCFastQC Report
Thu 28 Apr 2016
ERR1041404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286825
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146360.6400139932001793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT132450.5791873011708373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58170.25437014200911745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.18440414111267805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47000.023.1840421
GTATCAA59500.018.2201672
GACCGTA1059.351108E-615.85714226
TATACCG1153.5795785E-412.8695655
TATACTG3050.012.7377055
AACGCAG86600.012.6466517
ATCAACG87700.012.59354
TATCAAC87650.012.4951513
TCAACGC88900.012.40275
CGATGCG1205.124885E-412.33333433
ATAGCGG1050.002644948412.33333337
CAACGCA90150.012.1896836
GTAGATC1705.314416E-611.9705893
GTACTAG1552.722443E-511.93548430
TAGACAG4100.011.7317075
TAAGACG1451.9398086E-411.48275854
TCGTTAC1150.005221144811.2608713
CGAGAAC2155.213551E-711.1860473
CGGTCAA2655.3605618E-911.1698119
TATACAC3155.638867E-1111.158733
Produced by FastQC (version 0.11.5)