FastQCFastQC Report
Thu 28 Apr 2016
ERR1041403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941064
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148440.30042112387129577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135590.27441457953185794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84560.17113722874263518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51750.024.6309191
GTATCAA77000.016.457792
TAATACG3850.011.5324694
ATCAACG114850.011.5010892
AACGCAG115250.011.445125
TCAACGC116600.011.3602053
CAACGCA116600.011.3443394
CGCGCTA1150.005222601411.2608724
TTAGTAC3301.0913936E-1111.212123
AATACGC4000.011.15
TATCAAC118650.010.9924151
AACGGAT1200.007168009410.7916667
TAATACC6450.010.6124034
TATACTG6300.010.5714285
TGCGCTA10000.010.5455
TAGTACT5650.010.4778774
GTAATAC6550.010.4503813
GTATAGG7100.010.4225361
ACGCAGA127400.010.3681336
TATATCG2503.238074E-710.365
Produced by FastQC (version 0.11.5)