FastQCFastQC Report
Mon 16 Jan 2017
ERR1146687_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146687_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6898505
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC97950.044.20828694
GTACTAG103450.037.6784321
TAGGCAT125500.033.5515565
GCATCGT111400.033.155888
TAGTACT139500.033.0844154
CTGTGCG141550.032.4013529
CAACGCA115500.031.5738935
ACTGTGC154550.030.8617298
CTAGGCA141950.030.5268294
TCTAACG50600.030.094272
TACCCCG4850.030.0378135
TACTGTG163300.030.0138247
GTACTGT155200.029.9458896
CTAACGC51800.029.3971063
CGCCTAC51650.029.387017
CTAGTAC165750.029.3761943
TAACGCC53150.028.827074
ATCAACG129700.028.0834883
GTATCAA154700.027.8403031
GTCTAAC58750.027.5308341
Produced by FastQC (version 0.11.5)