##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146687_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6898505 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.521343392517654 31.0 31.0 34.0 30.0 34.0 2 31.594775534699185 33.0 31.0 34.0 28.0 34.0 3 31.71950908204024 33.0 31.0 34.0 28.0 34.0 4 35.18666392211066 37.0 35.0 37.0 32.0 37.0 5 35.33444913064497 37.0 35.0 37.0 33.0 37.0 6 35.468497449809774 37.0 35.0 37.0 33.0 37.0 7 35.41050937848128 37.0 35.0 37.0 33.0 37.0 8 35.408960782082495 37.0 35.0 37.0 33.0 37.0 9 36.99425948085853 39.0 37.0 39.0 33.0 39.0 10-11 36.83858836081151 39.0 37.0 39.0 32.5 39.0 12-13 36.79235319826542 39.0 37.0 39.0 32.0 39.0 14-15 37.99660143755784 40.0 38.0 41.0 32.0 41.0 16-17 37.992604629553796 40.0 38.0 41.0 32.0 41.0 18-19 37.92603317675351 40.0 38.0 41.0 32.0 41.0 20-21 37.98353048957709 40.0 38.0 41.0 32.0 41.0 22-23 37.874472077645805 40.0 38.0 41.0 32.0 41.0 24-25 37.79773914782986 40.0 37.0 41.0 32.0 41.0 26-27 37.82279102501194 40.0 37.0 41.0 32.0 41.0 28-29 37.77997769081851 40.0 37.0 41.0 32.0 41.0 30-31 37.622327446309015 40.0 37.0 41.0 31.0 41.0 32-33 37.62850994527075 40.0 37.0 41.0 31.0 41.0 34-35 37.55618702892873 40.0 37.0 41.0 31.0 41.0 36-37 37.41532556691631 40.0 37.0 41.0 31.0 41.0 38-39 37.29673835128045 40.0 36.0 41.0 31.0 41.0 40-41 37.19226267140489 40.0 36.0 41.0 30.0 41.0 42-43 36.99906117339916 39.5 35.5 41.0 30.0 41.0 44-45 36.803759220294836 39.0 35.0 41.0 30.0 41.0 46-47 36.7782660880872 39.0 35.0 41.0 30.0 41.0 48-49 36.58953244217406 39.0 35.0 41.0 30.0 41.0 50-51 36.366717209018475 39.0 35.0 40.5 29.5 41.0 52-53 36.10362172673645 38.0 35.0 40.0 29.0 41.0 54-55 35.8183546290102 38.0 34.5 40.0 29.0 41.0 56-57 35.52865889058572 37.0 34.0 40.0 28.0 41.0 58-59 35.158161007348696 36.5 34.0 40.0 28.0 41.0 60-61 34.85785376686688 36.0 34.0 39.0 28.0 41.0 62-63 34.49417047606691 35.0 33.0 39.0 27.0 41.0 64-65 34.30568804400374 35.0 33.0 38.5 27.0 40.5 66-67 34.02008841045995 35.0 33.0 37.5 27.0 40.0 68-69 33.863804403997676 35.0 33.0 37.0 27.0 40.0 70-71 33.78502255198771 35.0 33.0 37.0 27.0 39.5 72-73 33.56266024305266 35.0 33.0 36.0 27.0 39.0 74-75 33.33160365905366 35.0 33.0 36.0 27.0 39.0 76-77 33.10485800909038 35.0 33.0 35.0 26.5 37.5 78-79 32.89360607841844 35.0 33.0 35.0 26.0 37.0 80-81 32.7697694645434 35.0 33.0 35.0 26.0 36.5 82-83 32.569441060055766 35.0 33.0 35.0 26.0 36.0 84-85 32.463205578600004 35.0 33.0 35.0 26.0 36.0 86-87 32.35924588008561 35.0 33.0 35.0 25.0 36.0 88-89 32.290644494712986 35.0 33.0 35.0 25.0 35.5 90-91 32.18477728145446 35.0 32.5 35.0 25.0 35.0 92-93 32.068828101161046 35.0 32.0 35.0 25.0 35.0 94-95 32.04603135027082 35.0 32.0 35.0 25.0 35.0 96-97 31.963333649827028 35.0 32.0 35.0 25.0 35.0 98-99 31.893982029439712 35.0 32.0 35.0 25.0 35.0 100 30.912214023183285 34.0 30.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2904874790231631 1101 2 0.3433234055283627 1101 3 0.3784529256821614 1101 4 0.4334105548063718 1101 5 0.3269517763267231 1101 6 0.35281733712689345 1101 7 0.3967887477247203 1101 8 0.38438224822288447 1101 9 0.5591071045770519 1101 10-11 0.5483932042797193 1101 12-13 0.5107869320787657 1101 14-15 0.6558671914821375 1101 16-17 0.6490477180695606 1101 18-19 0.6686551905020011 1101 20-21 0.6101876577603349 1101 22-23 0.6634360690603103 1101 24-25 0.6560591672409899 1101 26-27 0.6467118479344478 1101 28-29 0.5619096572702134 1101 30-31 0.5832813786244699 1101 32-33 0.6250950812286078 1101 34-35 0.6081138996237385 1101 36-37 0.644025028326169 1101 38-39 0.5927841541067025 1101 40-41 0.5879956302365485 1101 42-43 0.5790046220363152 1101 44-45 0.5909805122818597 1101 46-47 0.6049109853793269 1101 48-49 0.4928091158578667 1101 50-51 0.5192504204015407 1101 52-53 0.5361738268987466 1101 54-55 0.5276059407697673 1101 56-57 0.48339998733702316 1101 58-59 0.4502445806982607 1101 60-61 0.44179801171378585 1101 62-63 0.4680902125146176 1101 64-65 0.49614320406797674 1101 66-67 0.4694594433597672 1101 68-69 0.45135340871854623 1101 70-71 0.46582507410296614 1101 72-73 0.4609748444324211 1101 74-75 0.44919842771354723 1101 76-77 0.4912424412179277 1101 78-79 0.4667198865676241 1101 80-81 0.3867704908092193 1101 82-83 0.4035974357441816 1101 84-85 0.4163769539870046 1101 86-87 0.4137529797966124 1101 88-89 0.4359989975403664 1101 90-91 0.4548385866775604 1101 92-93 0.4000336095433781 1101 94-95 0.46737118495588703 1101 96-97 0.47625003092909424 1101 98-99 0.45298085188213477 1101 100 0.5699575881247405 1102 1 0.3011493156653309 1102 2 0.3426515543911215 1102 3 0.3776050400997093 1102 4 0.4432447836406581 1102 5 0.3267867261030162 1102 6 0.3593535124717917 1102 7 0.3565563109415777 1102 8 0.3159493392329793 1102 9 0.42419014570140234 1102 10-11 0.5435978640979684 1102 12-13 0.4644199203026176 1102 14-15 0.6078197628842901 1102 16-17 0.590097968054863 1102 18-19 0.6115153676332312 1102 20-21 0.5771497477281571 1102 22-23 0.5853095231233567 1102 24-25 0.6062221968597825 1102 26-27 0.5360082886696134 1102 28-29 0.578224735606149 1102 30-31 0.6358574880082983 1102 32-33 0.5365357278648091 1102 34-35 0.5940826171757223 1102 36-37 0.6391042046849833 1102 38-39 0.5994397777371105 1102 40-41 0.5483000319092071 1102 42-43 0.5905386745164662 1102 44-45 0.5514675061438936 1102 46-47 0.5065118905775492 1102 48-49 0.5313535841670358 1102 50-51 0.5247922880323301 1102 52-53 0.511792743720072 1102 54-55 0.4359088451454838 1102 56-57 0.4933137954215283 1102 58-59 0.458812176121846 1102 60-61 0.4771783838280612 1102 62-63 0.5254934876323958 1102 64-65 0.5062199311093352 1102 66-67 0.5470562157856307 1102 68-69 0.5096846507462729 1102 70-71 0.48473717795271654 1102 72-73 0.5247663972710015 1102 74-75 0.46114783131540804 1102 76-77 0.496103066816886 1102 78-79 0.543379726417669 1102 80-81 0.4836205952043997 1102 82-83 0.48523082759718505 1102 84-85 0.5110667530142692 1102 86-87 0.4932445304301396 1102 88-89 0.55751879246003 1102 90-91 0.5633074357942789 1102 92-93 0.5388272813530364 1102 94-95 0.5362845409249104 1102 96-97 0.5291445239158747 1102 98-99 0.5000507411226529 1102 100 0.5534356488227274 1103 1 0.26660781349257334 1103 2 0.3360471460672869 1103 3 0.34094032783212214 1103 4 0.44194719014985395 1103 5 0.29874642513523497 1103 6 0.3673327784117646 1103 7 0.3528265718950081 1103 8 0.345390723238026 1103 9 0.4089759316199846 1103 10-11 0.5291218754480056 1103 12-13 0.47134306836020556 1103 14-15 0.6061787575904702 1103 16-17 0.582497901531049 1103 18-19 0.6147738762088579 1103 20-21 0.5148767791815558 1103 22-23 0.6023760588727214 1103 24-25 0.6263233769553125 1103 26-27 0.5596245023062494 1103 28-29 0.5496142738095742 1103 30-31 0.57289477071253 1103 32-33 0.5754695442623259 1103 34-35 0.5576298264842734 1103 36-37 0.6393543750291713 1103 38-39 0.6438243865296727 1103 40-41 0.6197289808685582 1103 42-43 0.6130456335328276 1103 44-45 0.5928100467787658 1103 46-47 0.5439146265425876 1103 48-49 0.6155108193653334 1103 50-51 0.4924688916321003 1103 52-53 0.4644329617670948 1103 54-55 0.4934721718716233 1103 56-57 0.5559301555977498 1103 58-59 0.5833838343011521 1103 60-61 0.5174976690505702 1103 62-63 0.45641931923646695 1103 64-65 0.44080640786198444 1103 66-67 0.437428791063887 1103 68-69 0.4349109115757628 1103 70-71 0.4196274459490894 1103 72-73 0.4361009516599452 1103 74-75 0.40030673041579945 1103 76-77 0.3593220226158351 1103 78-79 0.3992351891648269 1103 80-81 0.3263617133401553 1103 82-83 0.41031290913064566 1103 84-85 0.40616716652056795 1103 86-87 0.3456020666801365 1103 88-89 0.41416329743543656 1103 90-91 0.4154496008165651 1103 92-93 0.39265610407532137 1103 94-95 0.3602104929238408 1103 96-97 0.41514639068169146 1103 98-99 0.3554747956921851 1103 100 0.561534179908648 1104 1 0.19964997278983176 1104 2 0.31101466649318255 1104 3 0.2986022670258066 1104 4 0.4273080648192007 1104 5 0.3499510703164148 1104 6 0.28133142011531476 1104 7 0.2921454882529346 1104 8 0.310941466031764 1104 9 0.3935884733989852 1104 10-11 0.5023144588516573 1104 12-13 0.3791802242923765 1104 14-15 0.5321510539477998 1104 16-17 0.5078554724135884 1104 18-19 0.5049924231294227 1104 20-21 0.4686977104334531 1104 22-23 0.5221509634920167 1104 24-25 0.5173416526544656 1104 26-27 0.5222023295836635 1104 28-29 0.4450004884580707 1104 30-31 0.41813934289074695 1104 32-33 0.49593892282199903 1104 34-35 0.5055411063436921 1104 36-37 0.4487216068061315 1104 38-39 0.4447239951984301 1104 40-41 0.4900278717682838 1104 42-43 0.5076028910701282 1104 44-45 0.4599837502121247 1104 46-47 0.4584279386797263 1104 48-49 0.39293974853993774 1104 50-51 0.3322456856934295 1104 52-53 0.3532322377802046 1104 54-55 0.41424560171902414 1104 56-57 0.42277134569663133 1104 58-59 0.40363703266891804 1104 60-61 0.41681082512812395 1104 62-63 0.4682407566749234 1104 64-65 0.3988121357394263 1104 66-67 0.3675847346179566 1104 68-69 0.3876508871334252 1104 70-71 0.4007751714155674 1104 72-73 0.3664850842002423 1104 74-75 0.354779387803255 1104 76-77 0.3151160852577348 1104 78-79 0.2760407228873163 1104 80-81 0.2915165052760287 1104 82-83 0.37150917771253233 1104 84-85 0.35297242219075997 1104 86-87 0.3823467879556901 1104 88-89 0.3463777585197221 1104 90-91 0.34442497151282936 1104 92-93 0.387146563378316 1104 94-95 0.4341830239835929 1104 96-97 0.4575440028473352 1104 98-99 0.42187078464541017 1104 100 0.5305500674231354 1105 1 0.15669958973335696 1105 2 0.23587924374083613 1105 3 0.22584863796084775 1105 4 0.2582133326111773 1105 5 0.18515972396683367 1105 6 0.1554696671747422 1105 7 0.17242916850794643 1105 8 0.2370773124715484 1105 9 0.2816219306388277 1105 10-11 0.2898871870346511 1105 12-13 0.1901939047625376 1105 14-15 0.3675754302914811 1105 16-17 0.2686287288818576 1105 18-19 0.2955299289840809 1105 20-21 0.29663463189736206 1105 22-23 0.2950975275689558 1105 24-25 0.30710382911697565 1105 26-27 0.3168644264392739 1105 28-29 0.3491167422501533 1105 30-31 0.36466090626429093 1105 32-33 0.3728535845588823 1105 34-35 0.35201662887774177 1105 36-37 0.34028591337378344 1105 38-39 0.3476171307999252 1105 40-41 0.37561700625428784 1105 42-43 0.3637083035943931 1105 44-45 0.3145583643007228 1105 46-47 0.3445573884322144 1105 48-49 0.2205473709417447 1105 50-51 0.2710582788922551 1105 52-53 0.3187047113369772 1105 54-55 0.3538683833594618 1105 56-57 0.30993575752413705 1105 58-59 0.29885481587336216 1105 60-61 0.32851988674319443 1105 62-63 0.3331287877530542 1105 64-65 0.3370168160788438 1105 66-67 0.25289515186733524 1105 68-69 0.23161867861301033 1105 70-71 0.285341859983788 1105 72-73 0.2517389718634959 1105 74-75 0.2594335260379026 1105 76-77 0.2656730749419367 1105 78-79 0.2879891017036016 1105 80-81 0.24706025265901843 1105 82-83 0.286916143150556 1105 84-85 0.21531041553423336 1105 86-87 0.2024398543769692 1105 88-89 0.25413290400504707 1105 90-91 0.2190431769979284 1105 92-93 0.2318576697817889 1105 94-95 0.27651476752360793 1105 96-97 0.14661710796320193 1105 98-99 0.21987660910641083 1105 100 0.3585541727227053 1106 1 0.09352167905727526 1106 2 0.17709509694425307 1106 3 0.14185952724633566 1106 4 0.24810203797970587 1106 5 0.11290485297918451 1106 6 0.20109428612364866 1106 7 0.16430815222389583 1106 8 0.17625448229193097 1106 9 0.25205568225078423 1106 10-11 0.31416135629707753 1106 12-13 0.23103221716345956 1106 14-15 0.3275310389940387 1106 16-17 0.26016516845131576 1106 18-19 0.27939481704281377 1106 20-21 0.2834656308886139 1106 22-23 0.31665683482268037 1106 24-25 0.37909801497013973 1106 26-27 0.36493708387572354 1106 28-29 0.369191358982043 1106 30-31 0.44660849765188715 1106 32-33 0.32189002874714134 1106 34-35 0.31996550706333693 1106 36-37 0.3345542721844197 1106 38-39 0.3463685672470689 1106 40-41 0.33351410767946277 1106 42-43 0.3085436387161238 1106 44-45 0.33187596322621005 1106 46-47 0.3494775940242931 1106 48-49 0.2708072801176371 1106 50-51 0.33245726294076405 1106 52-53 0.23571535859729664 1106 54-55 0.2991668040989808 1106 56-57 0.2587747710590662 1106 58-59 0.3190858621611383 1106 60-61 0.3156579944388582 1106 62-63 0.3324472975061141 1106 64-65 0.2110439626204652 1106 66-67 0.24481178804991544 1106 68-69 0.2525028095209194 1106 70-71 0.2175527876976986 1106 72-73 0.1879901080885702 1106 74-75 0.2626332605492152 1106 76-77 0.2447536332995668 1106 78-79 0.2526483542707325 1106 80-81 0.20496212530573388 1106 82-83 0.2547197969917363 1106 84-85 0.24872178813107126 1106 86-87 0.245284010485058 1106 88-89 0.28228043682625525 1106 90-91 0.2537741503086508 1106 92-93 0.29126210309184586 1106 94-95 0.29035897259097965 1106 96-97 0.30577372411865866 1106 98-99 0.25729505493525906 1106 100 0.2705088216383942 1107 1 0.10560304318206093 1107 2 0.14717439248959252 1107 3 0.08541394607748742 1107 4 0.22901214356604527 1107 5 0.13550753103343993 1107 6 0.17054623743503328 1107 7 0.11330464146583097 1107 8 0.159224232612587 1107 9 0.1623351902035779 1107 10-11 0.26491780291878797 1107 12-13 0.15674318902814832 1107 14-15 0.26635278113749905 1107 16-17 0.1679846346024263 1107 18-19 0.1061303392412043 1107 20-21 0.15311465913491418 1107 22-23 0.2715331674852166 1107 24-25 0.32072174578010504 1107 26-27 0.20286091547865936 1107 28-29 0.3097889439745032 1107 30-31 0.43450752988690766 1107 32-33 0.3762679738888579 1107 34-35 0.3108681832446294 1107 36-37 0.3077650723611711 1107 38-39 0.29388312950527506 1107 40-41 0.3278630566371632 1107 42-43 0.355763286197444 1107 44-45 0.30080762033037445 1107 46-47 0.3133615565678838 1107 48-49 0.3163386976217666 1107 50-51 0.2660146715698346 1107 52-53 0.2530811315849135 1107 54-55 0.2550949074363942 1107 56-57 0.25505851467453056 1107 58-59 0.2240665047803887 1107 60-61 0.2177668910646844 1107 62-63 0.2512937904592789 1107 64-65 0.2353683896857035 1107 66-67 0.19991556733484117 1107 68-69 0.2701082241767594 1107 70-71 0.2737067282999206 1107 72-73 0.2598351047553962 1107 74-75 0.24589873032554266 1107 76-77 0.23243218354586048 1107 78-79 0.2723522879707474 1107 80-81 0.2216772045007005 1107 82-83 0.19069781816173048 1107 84-85 0.14861040948738236 1107 86-87 0.1718951265675983 1107 88-89 0.23359205631862778 1107 90-91 0.18135973240704573 1107 92-93 0.18483681231492 1107 94-95 0.1622685794152119 1107 96-97 0.200694494473737 1107 98-99 0.2018816962961658 1107 100 0.2709796419737387 1108 1 0.018514870050260868 1108 2 0.05809265575921074 1108 3 0.02974995633725186 1108 4 0.14296531231062204 1108 5 0.07200014630078755 1108 6 0.09278322967018227 1108 7 0.11136872207919168 1108 8 0.14067112796808345 1108 9 0.15909078511775476 1108 10-11 0.26406610652797724 1108 12-13 0.11166047770799992 1108 14-15 0.22063138440578456 1108 16-17 0.26027110878798254 1108 18-19 0.18457704834072786 1108 20-21 0.14531105363298735 1108 22-23 0.20598299681014964 1108 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-0.8402655001881598 2215 30-31 -3.5426864088886276 2215 32-33 -1.390497171082039 2215 34-35 -1.139693813425481 2215 36-37 -0.942247409757698 2215 38-39 -0.7665765971693475 2215 40-41 -0.7939888283278975 2215 42-43 -0.7733766743484907 2215 44-45 -0.7202037565596058 2215 46-47 -0.9084560727409254 2215 48-49 -0.9450617985919934 2215 50-51 -0.9178201589573334 2215 52-53 -0.8908384873670272 2215 54-55 -0.7895153392274494 2215 56-57 -0.8507931431027487 2215 58-59 -0.8603988172186448 2215 60-61 -0.8200830086490782 2215 62-63 -0.8008139085974975 2215 64-65 -0.8285403767573456 2215 66-67 -0.782261279523425 2215 68-69 -0.7114885384965604 2215 70-71 -0.8165669644457836 2215 72-73 -0.7398813687026049 2215 74-75 -0.8630032576335012 2215 76-77 -0.7738606122902567 2215 78-79 -0.7356572615106103 2215 80-81 -0.6927521826999126 2215 82-83 -0.6818680902327294 2215 84-85 -0.698351936450905 2215 86-87 -0.6638004890386142 2215 88-89 -0.6174671076039573 2215 90-91 -0.7535381915986434 2215 92-93 -0.749720350671172 2215 94-95 -0.7239429967924167 2215 96-97 -0.9933736839822558 2215 98-99 -0.846381873385095 2215 100 -0.4029437587630369 2216 1 -0.34935275656065556 2216 2 -0.5326232528332326 2216 3 -0.5141566728182134 2216 4 -0.3651510353937226 2216 5 -0.30575705685618715 2216 6 -0.33092198797718453 2216 7 -0.4392837875383293 2216 8 -0.4473206604882023 2216 9 -0.5792560209192388 2216 10-11 -0.913901021586426 2216 12-13 -0.5502832687979833 2216 14-15 -0.6299921118051444 2216 16-17 -0.6442411473064951 2216 18-19 -0.9374997425627498 2216 20-21 -0.9411921306011806 2216 22-23 -0.8431896092955355 2216 24-25 -0.8762908779676053 2216 26-27 -1.254392598543312 2216 28-29 -1.1683508445922897 2216 30-31 -1.2554898179069482 2216 32-33 -1.4803687835951678 2216 34-35 -1.117987898663607 2216 36-37 -1.2026281034780055 2216 38-39 -1.10711821785452 2216 40-41 -0.9910029154603421 2216 42-43 -0.9897369032757553 2216 44-45 -0.9129125648514744 2216 46-47 -1.025473837016115 2216 48-49 -0.9972040474339181 2216 50-51 -1.0725624202996187 2216 52-53 -1.0654825369213157 2216 54-55 -1.0824159412821288 2216 56-57 -0.9739653581111085 2216 58-59 -0.8946270343812515 2216 60-61 -0.9607667933719881 2216 62-63 -1.066509450264988 2216 64-65 -0.9775079339230786 2216 66-67 -1.003834157711566 2216 68-69 -1.0280020138273542 2216 70-71 -0.986362898855802 2216 72-73 -0.8683743170138953 2216 74-75 -0.9265803121526801 2216 76-77 -0.8992551307456367 2216 78-79 -0.8166286467044586 2216 80-81 -0.8742920863755685 2216 82-83 -0.976303083690695 2216 84-85 -0.9945038224079923 2216 86-87 -0.9604367500989497 2216 88-89 -1.0348924413690241 2216 90-91 -1.060923810570113 2216 92-93 -1.0018838143276199 2216 94-95 -1.119701644617134 2216 96-97 -1.1515963450937008 2216 98-99 -1.1583764830875758 2216 100 -0.7056681759820336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 1 2.0 2 21.0 3 36.0 4 52.0 5 40.0 6 61.0 7 301.0 8 901.0 9 1336.0 10 1656.0 11 2813.0 12 4723.0 13 6807.0 14 9744.0 15 12875.0 16 15964.0 17 19414.0 18 22150.0 19 25604.0 20 29550.0 21 34571.0 22 40294.0 23 47068.0 24 54759.0 25 63782.0 26 73311.0 27 84256.0 28 99396.0 29 119904.0 30 144022.0 31 173605.0 32 217970.0 33 285430.0 34 412851.0 35 613235.0 36 1064842.0 37 1816451.0 38 1169546.0 39 229062.0 40 100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.149114263522826 19.016323774810378 11.384536615541707 24.45002534612509 2 16.75695117243251 18.788928805398776 38.990613088501334 25.46350693366738 3 19.72720772815757 24.809828825647333 27.570616161563443 27.89234728463165 4 12.960482328183343 15.364642681312334 38.0899569664633 33.58491802404102 5 14.015004629420908 38.188801222799164 33.76183684288425 14.034357304895675 6 32.175089506962976 36.56937474577239 16.87922003332393 14.376315713940704 7 28.775636230800007 30.56105438330985 22.310942763871253 18.35236662201889 8 27.763168099246272 33.888491453446775 18.56747837721673 19.780862070090215 9 26.566519691855905 14.390630026030443 20.04486218012974 38.99798810198391 10-11 27.38012403920641 24.370541036381432 26.10566642081484 22.143668503597322 12-13 27.16861942648418 22.03065760313008 26.266356038054443 24.5343669323313 14-15 24.523160071720053 23.29088297451804 23.484229662466667 28.70172729129524 16-17 23.804254862679496 26.094207982651202 24.65926453150413 25.44227262316517 18-19 23.08700680121724 27.00717811636688 26.152117212729475 23.753697869686402 20-21 25.06182922305444 26.260093409565094 25.251676575432956 23.42640079194751 22-23 25.72353834389734 25.555108593628255 25.092474006747917 23.628879055726486 24-25 25.15780766896347 25.358128640593385 25.758357446311326 23.725706244131818 26-27 24.956065052280522 25.708630877871208 24.34477383957449 24.990530230273777 28-29 23.826444262272506 26.149100242163044 25.552100570507424 24.472354925057026 30-31 24.552930893278564 26.02168094084547 25.573279960789648 23.852108205086314 32-33 24.831131207445754 25.931063406235943 24.500678229549063 24.737127156769244 34-35 24.85919879123658 26.17360434061226 25.318732998955756 23.6484638691954 36-37 24.490188078055823 25.21245953785905 24.570929334837448 25.72642304924767 38-39 24.71942624175914 25.680597127004738 25.176632047940178 24.423344583295943 40-41 24.302937335049844 24.50028859368872 26.33153750235662 24.865236568904812 42-43 24.321095780623505 24.703153832860313 25.61290215950331 25.36284822701287 44-45 24.912122562709634 25.2852935709557 25.525278283384363 24.277305582950298 46-47 24.553712271821507 25.658249570262388 24.707063645242034 25.08097451267407 48-49 25.37529792756162 25.44826421786045 24.851260047726406 24.325177806851524 50-51 24.531536709219004 24.89977138655043 25.720847145637553 24.847844758593013 52-53 24.587961551608704 25.17728750855019 25.25683915422819 24.977911785612918 54-55 24.092957917399787 25.15895795466136 25.365919185441655 25.382164942497198 56-57 25.477520331774546 24.84390981891147 24.68921508005489 24.989354769259094 58-59 23.91581409351607 25.416842067262053 25.68972395238634 24.977619886835534 60-61 23.96585580003114 25.83590266120799 25.133332966044858 25.064908572716014 62-63 24.551610057504384 25.3149620853802 24.590680853932486 25.54274700318293 64-65 23.765531455939417 25.441219078348247 25.251613373693438 25.541636092018894 66-67 24.565867464786038 25.36394092509396 25.0551355351206 25.015056074999404 68-69 24.071505615967542 25.05163164552898 25.748877216021963 25.127985522481524 70-71 23.732339930407868 24.95225362595547 25.43782127503622 25.877585168600444 72-73 24.339127825304114 24.85054936770886 26.0314181210885 24.778904685898524 74-75 24.806585060168302 24.91016978761869 25.56329722727823 24.71994792493478 76-77 24.525551752155458 24.95352305971686 24.763976444289902 25.756948743837775 78-79 24.702346132250305 25.04796893686048 24.515261841894095 25.73442308899512 80-81 24.023484573968425 25.69089918393599 25.468328364481774 24.81728787761381 82-83 24.965765786662068 24.64732889054475 26.695336521664753 23.691568801128433 84-85 24.744290731431512 24.973987988880474 25.660767421378633 24.620953858309385 86-87 23.40314504628861 25.754605030963575 26.03124866127026 24.811001261477557 88-89 24.935214443074713 24.20679507729072 25.724898391906176 25.133092087728386 90-91 24.236471134973215 24.821269212041965 25.228077701581324 25.714181951403493 92-93 24.6311712117109 24.681802445840166 25.82979410013111 24.857232242317824 94-95 23.782955027972605 24.723814833838045 26.548242468576756 24.94498766961259 96-97 24.054059128095556 24.50029208357468 26.007666586762596 25.437982201567166 98-99 23.400782154264387 24.943366591899647 25.951141163535652 25.704710090300313 100 24.16450077286222 24.288277897884527 25.136493455231836 26.410727874021422 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 539.0 1 391.5 2 287.5 3 348.5 4 346.0 5 306.5 6 267.5 7 256.0 8 290.0 9 295.5 10 262.0 11 239.5 12 246.5 13 249.0 14 250.0 15 291.5 16 315.0 17 351.0 18 422.5 19 499.0 20 593.5 21 749.0 22 1040.0 23 1403.0 24 1809.5 25 2324.5 26 3084.0 27 4037.5 28 5486.0 29 8568.0 30 13600.0 31 19406.5 32 28256.0 33 38325.0 34 47451.0 35 58450.0 36 72213.5 37 96575.5 38 118428.0 39 131914.5 40 137756.0 41 139463.0 42 148513.0 43 167631.5 44 197911.0 45 247085.0 46 298129.5 47 341196.0 48 374853.5 49 382568.5 50 372049.5 51 394270.5 52 413410.5 53 398832.0 54 381951.0 55 332900.5 56 293696.5 57 247020.0 58 197470.5 59 193400.0 60 162689.5 61 113942.5 62 87448.5 63 73826.5 64 54405.5 65 27684.5 66 16217.5 67 11409.5 68 8338.5 69 6089.0 70 4533.0 71 3417.5 72 2471.5 73 1838.5 74 1311.0 75 947.0 76 647.5 77 333.0 78 186.5 79 115.5 80 88.5 81 79.5 82 60.5 83 48.0 84 42.5 85 38.5 86 31.0 87 28.0 88 26.0 89 20.5 90 17.5 91 18.5 92 18.5 93 14.5 94 16.5 95 17.0 96 13.0 97 11.5 98 8.5 99 11.5 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05740374182522155 2 0.007189963622553002 3 0.007378410249757012 4 0.00911791757779403 5 0.0033920392896721824 6 0.0023048472096490473 7 9.422331360200508E-4 8 0.001594548384033932 9 6.378193536135729E-4 10-11 5.943316704126474E-4 12-13 2.971658352063237E-4 14-15 2.3193431040493558E-4 16-17 6.523152480138812E-4 18-19 0.0015583086480331606 20-21 9.85720819220976E-4 22-23 0.0011596715520246778 24-25 0.001101687974423444 26-27 0.0034500228672734163 28-29 0.003768932544080203 30-31 0.004732909521700716 32-33 0.0057693659713227725 34-35 0.003123865243266476 36-37 0.007893014500967964 38-39 0.005914324915325857 40-41 0.005776613918522926 42-43 0.014394423139506313 44-45 0.020279756266031554 46-47 0.018453273571592686 48-49 0.021519155237257927 50-51 0.018453273571592686 52-53 0.025041657576532886 54-55 0.019127332661207027 56-57 0.015220689120323897 58-59 0.017373329438769704 60-61 0.016104938678742714 62-63 0.011161838688237525 64-65 0.01115459074103737 66-67 0.013872570941095208 68-69 0.005240265825711513 70-71 0.008683040745784775 72-73 0.008335139280177371 74-75 0.011161838688237525 76-77 0.01057475496502503 78-79 0.011770666253050479 80-81 0.010154374027416084 82-83 0.006305714064134185 84-85 0.010582002912225184 86-87 0.009299116257797885 88-89 0.008052469339371357 90-91 0.013198511851480863 92-93 0.00853808180178169 94-95 0.0073349225665560866 96-97 0.009429579307400661 98-99 0.009175901155395263 100 0.25496828660702575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6898505.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.495299041133606 #Duplication Level Percentage of deduplicated Percentage of total 1 70.88754301733248 42.17475570107418 2 15.784539776459678 18.782118284542733 3 5.983972869279651 10.680547659354694 4 2.867035603976178 6.823005624805592 5 1.5343746220012235 4.5644038488544565 6 0.9171379230194482 3.273923699520375 7 0.5520693960799562 2.299187366786452 8 0.36335148607804163 1.7294164257002704 9 0.2668205653807625 1.4287112394887522 >10 0.8267866700736494 7.419801067747817 >50 0.013280330543799642 0.5329442707636869 >100 0.0030475288785498066 0.26991673702042135 >500 4.02108966078282E-5 0.02126807434053016 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4495894400308472E-5 2 0.0 0.0 0.0 0.0 1.4495894400308472E-5 3 0.0 0.0 0.0 0.0 1.4495894400308472E-5 4 0.0 0.0 0.0 0.0 1.4495894400308472E-5 5 0.0 0.0 0.0 0.0 2.8991788800616944E-5 6 0.0 0.0 0.0 0.0 2.8991788800616944E-5 7 0.0 0.0 0.0 0.0 2.8991788800616944E-5 8 0.0 0.0 0.0 0.0 2.8991788800616944E-5 9 0.0 0.0 0.0 0.0 2.8991788800616944E-5 10-11 0.0 0.0 0.0 0.0 2.8991788800616944E-5 12-13 0.0 0.0 0.0 1.4495894400308472E-5 1.9569457440416437E-4 14-15 7.247947200154236E-6 0.0 0.0 2.174384160046271E-5 2.536781520053983E-4 16-17 1.4495894400308472E-5 0.0 0.0 3.623973600077118E-5 3.0441378240647795E-4 18-19 1.4495894400308472E-5 0.0 0.0 5.073563040107965E-5 3.3340557120709486E-4 20-21 1.4495894400308472E-5 0.0 0.0 6.523152480138812E-5 3.623973600077118E-4 22-23 1.4495894400308472E-5 0.0 0.0 1.6670278560354743E-4 3.623973600077118E-4 24-25 1.4495894400308472E-5 0.0 0.0 3.551494128075576E-4 3.623973600077118E-4 26-27 1.4495894400308472E-5 0.0 0.0 7.03050878414961E-4 3.9863709600848304E-4 28-29 1.4495894400308472E-5 0.0 0.0 0.0030803775600655504 4.203809376089457E-4 30-31 1.4495894400308472E-5 0.0 0.0 0.012459221237065133 4.203809376089457E-4 32-33 1.4495894400308472E-5 0.0 0.0 0.030020997303038848 4.203809376089457E-4 34-35 1.4495894400308472E-5 0.0 0.0 0.055164126140373894 4.203809376089457E-4 36-37 1.4495894400308472E-5 0.0 0.0 0.09939834790291519 4.203809376089457E-4 38-39 1.4495894400308472E-5 0.0 0.0 0.1659345032003311 4.348768320092542E-4 40-41 1.4495894400308472E-5 0.0 0.0 0.23915326581628918 4.348768320092542E-4 42-43 1.4495894400308472E-5 0.0 0.0 0.3169454831155446 4.4212477920940843E-4 44-45 1.4495894400308472E-5 0.0 0.0 0.41182111196556354 4.5662067360971686E-4 46-47 1.4495894400308472E-5 0.0 0.0 0.5311948023521038 4.638686208098711E-4 48-49 1.4495894400308472E-5 0.0 0.0 0.6730588729007227 4.638686208098711E-4 50-51 1.4495894400308472E-5 0.0 0.0 0.8228811894751109 4.638686208098711E-4 52-53 1.4495894400308472E-5 0.0 0.0 0.9689708132414196 5.798357760123389E-4 54-55 1.4495894400308472E-5 0.0 0.0 1.1342312573521365 5.943316704126474E-4 56-57 1.4495894400308472E-5 0.0 0.0 1.3402106688333197 5.943316704126474E-4 58-59 1.4495894400308472E-5 0.0 0.0 1.5863509557505575 5.943316704126474E-4 60-61 1.4495894400308472E-5 0.0 0.0 1.8487991238681425 5.943316704126474E-4 62-63 1.4495894400308472E-5 0.0 0.0 2.1200462998867144 6.233234592132644E-4 64-65 1.4495894400308472E-5 0.0 0.0 2.4064199417120085 6.305714064134186E-4 66-67 1.4495894400308472E-5 0.0 0.0 2.731287431117322 6.523152480138812E-4 68-69 1.4495894400308472E-5 0.0 0.0 3.09725802909471 6.523152480138812E-4 70-71 1.4495894400308472E-5 0.0 0.0 3.5108911278603117 6.523152480138812E-4 72-73 1.4495894400308472E-5 0.0 7.247947200154236E-6 3.9436080716039204 7.175467728152694E-4 74-75 1.4495894400308472E-5 0.0 1.4495894400308472E-5 4.366395327683317 7.900262448168117E-4 76-77 1.4495894400308472E-5 0.0 1.4495894400308472E-5 4.848854933061584 8.117700864172744E-4 78-79 1.4495894400308472E-5 0.0 1.4495894400308472E-5 5.371816067394312 8.262659808175829E-4 80-81 1.4495894400308472E-5 0.0 1.4495894400308472E-5 5.929596340076582 8.407618752178914E-4 82-83 1.4495894400308472E-5 0.0 1.4495894400308472E-5 6.523420654185218 8.407618752178914E-4 84-85 1.4495894400308472E-5 0.0 1.4495894400308472E-5 7.099878886802285 8.407618752178914E-4 86-87 1.4495894400308472E-5 0.0 1.4495894400308472E-5 7.7040387736183416 8.625057168183542E-4 88 1.4495894400308472E-5 0.0 1.4495894400308472E-5 8.193282457575952 8.697536640185084E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 9795 0.0 44.208286 94 GTACTAG 10345 0.0 37.678432 1 TAGGCAT 12550 0.0 33.551556 5 GCATCGT 11140 0.0 33.15588 8 TAGTACT 13950 0.0 33.084415 4 CTGTGCG 14155 0.0 32.401352 9 CAACGCA 11550 0.0 31.573893 5 ACTGTGC 15455 0.0 30.861729 8 CTAGGCA 14195 0.0 30.526829 4 TCTAACG 5060 0.0 30.09427 2 TACCCCG 485 0.0 30.037813 5 TACTGTG 16330 0.0 30.013824 7 GTACTGT 15520 0.0 29.945889 6 CTAACGC 5180 0.0 29.397106 3 CGCCTAC 5165 0.0 29.38701 7 CTAGTAC 16575 0.0 29.376194 3 TAACGCC 5315 0.0 28.82707 4 ATCAACG 12970 0.0 28.083488 3 GTATCAA 15470 0.0 27.840303 1 GTCTAAC 5875 0.0 27.530834 1 >>END_MODULE