FastQCFastQC Report
Mon 16 Jan 2017
ERR1146687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6898505
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70820.1026599241429846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC103150.042.8518794
GTACTAG104800.035.7793051
CAGTACG13900.033.8175439
TACCCCG5300.033.6985365
GTATCAA185900.033.6594541
TAGTACT143550.032.8040854
CTGTGCG145050.032.5042429
CAACGCA178200.031.5974835
TAGGCAT132850.031.3455245
GGTATCA120250.031.2996671
ACTGTGC159250.030.9288068
GTACTGT156800.030.4824526
ATCAACG187750.029.9673443
TACTGTG169350.029.8055557
CTAACGC48200.029.182413
TCTAACG49350.028.8861942
CTAGGCA148650.028.8303894
TAACGCC48950.028.7352854
CTAGTAC170900.028.6828753
CGCCTAC49500.028.6734477
Produced by FastQC (version 0.11.5)