##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146687_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6898505 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07102104006593 33.0 31.0 34.0 30.0 34.0 2 32.16515940772675 33.0 31.0 34.0 30.0 34.0 3 32.33062743304527 34.0 31.0 34.0 30.0 34.0 4 35.94146427378106 37.0 35.0 37.0 35.0 37.0 5 35.834563285813374 37.0 35.0 37.0 35.0 37.0 6 35.865725399923605 37.0 35.0 37.0 35.0 37.0 7 35.79174096416543 37.0 35.0 37.0 33.0 37.0 8 35.798799739943654 37.0 35.0 37.0 33.0 37.0 9 37.51493809165899 39.0 37.0 39.0 35.0 39.0 10-11 37.455398089876 39.0 37.0 39.0 34.0 39.0 12-13 37.414564097583465 39.0 37.0 39.0 34.0 39.0 14-15 38.64384478955948 40.0 38.0 41.0 34.0 41.0 16-17 38.628528934892415 40.0 38.0 41.0 34.0 41.0 18-19 38.66141055199641 40.0 38.0 41.0 34.0 41.0 20-21 38.649878488165186 40.0 38.0 41.0 34.0 41.0 22-23 38.60397578895717 40.0 38.0 41.0 34.0 41.0 24-25 38.5237908793282 40.0 38.0 41.0 34.0 41.0 26-27 38.389074516869954 40.0 38.0 41.0 34.0 41.0 28-29 38.32446044469055 40.0 38.0 41.0 34.0 41.0 30-31 38.21908000356599 40.0 38.0 41.0 33.0 41.0 32-33 38.11381987836495 40.0 37.0 41.0 33.0 41.0 34-35 38.0270425258806 40.0 37.0 41.0 33.0 41.0 36-37 38.02850008806256 40.0 37.0 41.0 33.0 41.0 38-39 37.94004099438936 40.0 37.0 41.0 33.0 41.0 40-41 37.82501295570562 40.0 37.0 41.0 33.0 41.0 42-43 37.68653679311677 40.0 36.5 41.0 32.5 41.0 44-45 37.48673415471903 40.0 36.0 41.0 32.0 41.0 46-47 37.272847377801426 39.5 36.0 41.0 31.5 41.0 48-49 37.02433317073772 39.0 35.0 41.0 31.0 41.0 50-51 36.78810278458884 39.0 35.0 41.0 31.0 41.0 52-53 36.560466651832535 38.5 35.0 41.0 31.0 41.0 54-55 36.31347596327031 38.0 35.0 40.0 30.5 41.0 56-57 36.05428654469338 37.5 35.0 40.0 30.0 41.0 58-59 35.729847264008654 37.0 35.0 40.0 30.0 41.0 60-61 35.52221336362009 36.0 34.0 39.0 30.0 41.0 62-63 35.254016703619115 36.0 34.0 39.0 30.0 41.0 64-65 34.964105701162794 35.0 34.0 39.0 29.5 41.0 66-67 34.684213318682815 35.0 34.0 37.5 29.0 40.5 68-69 34.40205501046966 35.0 34.0 37.0 29.0 40.0 70-71 34.18304813869092 35.0 34.0 37.0 29.0 39.5 72-73 33.94633279239488 35.0 34.0 36.0 29.0 39.0 74-75 33.71198861202536 35.0 33.5 36.0 29.0 39.0 76-77 33.50544878926666 35.0 33.0 35.0 29.0 37.5 78-79 33.3220396303257 35.0 33.0 35.0 29.0 37.0 80-81 33.233963953059394 35.0 33.0 35.0 29.0 36.5 82-83 33.12667773669803 35.0 33.0 35.0 29.0 36.0 84-85 33.06505039860086 35.0 33.0 35.0 29.0 36.0 86-87 32.98263471578262 35.0 33.0 35.0 29.0 36.0 88-89 32.92637056869568 35.0 33.0 35.0 29.0 35.5 90-91 32.89466623565541 35.0 33.0 35.0 29.0 35.0 92-93 32.85846476881585 35.0 33.0 35.0 29.0 35.0 94-95 32.839282714153285 35.0 33.0 35.0 29.0 35.0 96-97 32.76253188190775 35.0 33.0 35.0 29.0 35.0 98-99 32.69332398831341 35.0 33.0 35.0 29.0 35.0 100 31.394443143840586 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.387622157067959 1101 2 0.42976451395739446 1101 3 0.35031215424955775 1101 4 0.25486608830434676 1101 5 0.22481415740470823 1101 6 0.27755153795300913 1101 7 0.26980464719872543 1101 8 0.2709438158476729 1101 9 0.35944536433654406 1101 10-11 0.38253115519259495 1101 12-13 0.3634578675708511 1101 14-15 0.5120767252671783 1101 16-17 0.523331299981237 1101 18-19 0.49757497447488674 1101 20-21 0.5226680336185581 1101 22-23 0.5289607146416913 1101 24-25 0.5285672380664153 1101 26-27 0.5742085632587788 1101 28-29 0.5593023101437069 1101 30-31 0.5965935253282453 1101 32-33 0.6453001201415063 1101 34-35 0.6519903940777212 1101 36-37 0.5479262894187968 1101 38-39 0.5963185776917967 1101 40-41 0.5752916608037779 1101 42-43 0.5726657906838497 1101 44-45 0.5704280925580036 1101 46-47 0.5481356612209254 1101 48-49 0.49602309455065097 1101 50-51 0.5110457275223581 1101 52-53 0.5459152260134772 1101 54-55 0.5643631884181914 1101 56-57 0.5514613213812467 1101 58-59 0.5163965035249021 1101 60-61 0.5275029053582543 1101 62-63 0.5271428513037577 1101 64-65 0.5633026404716972 1101 66-67 0.49744569185025966 1101 68-69 0.49927836882692844 1101 70-71 0.4427583047630037 1101 72-73 0.488205596741615 1101 74-75 0.464166199785339 1101 76-77 0.45283044579584697 1101 78-79 0.4372682416962448 1101 80-81 0.5147568621862106 1101 82-83 0.45056122593341286 1101 84-85 0.43238841106335 1101 86-87 0.38714730404649345 1101 88-89 0.39680532701800075 1101 90-91 0.3909075799899213 1101 92-93 0.4076124370466374 1101 94-95 0.39282733771327827 1101 96-97 0.42036424162927943 1101 98-99 0.4631691027011158 1101 100 0.8276233851133057 1102 1 0.34915716554424847 1102 2 0.4120452125407681 1102 3 0.320880199165515 1102 4 0.2519467445410868 1102 5 0.2606702118871027 1102 6 0.20935661359781932 1102 7 0.28043604156285085 1102 8 0.2794303052962164 1102 9 0.3342052115476619 1102 10-11 0.33041028133547456 1102 12-13 0.38357759538020275 1102 14-15 0.4642528593900934 1102 16-17 0.44050345601259266 1102 18-19 0.4663934747237164 1102 20-21 0.4871484426447381 1102 22-23 0.545029554807094 1102 24-25 0.5289995573511774 1102 26-27 0.5215368440141717 1102 28-29 0.5278920322158456 1102 30-31 0.5540548477531004 1102 32-33 0.5946358186072516 1102 34-35 0.5629395838971263 1102 36-37 0.5554700006773814 1102 38-39 0.5444333522730389 1102 40-41 0.5613523865804737 1102 42-43 0.505957506933207 1102 44-45 0.5337836742680366 1102 46-47 0.5272474272730818 1102 48-49 0.5416822234592615 1102 50-51 0.5297138679190709 1102 52-53 0.5095985412351354 1102 54-55 0.4907020820218264 1102 56-57 0.44052581777886957 1102 58-59 0.47778826115362705 1102 60-61 0.48408798508034323 1102 62-63 0.4800563678965304 1102 64-65 0.4646882313617482 1102 66-67 0.4881415336748418 1102 68-69 0.5140163301816472 1102 70-71 0.45403149735239623 1102 72-73 0.44601191286570696 1102 74-75 0.4933714362342272 1102 76-77 0.38640126578520295 1102 78-79 0.4373710855685715 1102 80-81 0.4240506007209177 1102 82-83 0.38450372656684806 1102 84-85 0.38423097118371885 1102 86-87 0.44997444838580236 1102 88-89 0.43410341919808104 1102 90-91 0.4134447074782912 1102 92-93 0.40965030215826204 1102 94-95 0.40128329778875127 1102 96-97 0.403243272889803 1102 98-99 0.4416908869534595 1102 100 0.6936252379219496 1103 1 0.30193426011618385 1103 2 0.35728310373932004 1103 3 0.25790336048913787 1103 4 0.21914874871197298 1103 5 0.20267325790925383 1103 6 0.23297087041457587 1103 7 0.2370307908623488 1103 8 0.2528541494168337 1103 9 0.28717040290931095 1103 10-11 0.3327745433973206 1103 12-13 0.3447957452114423 1103 14-15 0.4319643226265697 1103 16-17 0.416937373277122 1103 18-19 0.37576681142681423 1103 20-21 0.37617654144857227 1103 22-23 0.3811181308845377 1103 24-25 0.4307214055316493 1103 26-27 0.45873728918463996 1103 28-29 0.491760724840681 1103 30-31 0.4149417180645969 1103 32-33 0.4329284194234191 1103 34-35 0.43257900695076756 1103 36-37 0.37666544252167 1103 38-39 0.3638089717981714 1103 40-41 0.33946373826611875 1103 42-43 0.3883856759081894 1103 44-45 0.40858738707756004 1103 46-47 0.4398707593766389 1103 48-49 0.4173827448044989 1103 50-51 0.47563158255357507 1103 52-53 0.4059765362185743 1103 54-55 0.39041359028533407 1103 56-57 0.35001490945617064 1103 58-59 0.37329256631875296 1103 60-61 0.3979441338845149 1103 62-63 0.4201797976393564 1103 64-65 0.41482549521595047 1103 66-67 0.45636302083212854 1103 68-69 0.3974996688891821 1103 70-71 0.4316090043654768 1103 72-73 0.41143724562191863 1103 74-75 0.37153500878536505 1103 76-77 0.3727780067900355 1103 78-79 0.4123199984482113 1103 80-81 0.35434667927000874 1103 82-83 0.364142834108506 1103 84-85 0.37584593745864225 1103 86-87 0.34007314957383983 1103 88-89 0.3227987375811523 1103 90-91 0.3186461243611305 1103 92-93 0.3164173766826792 1103 94-95 0.3308769788663284 1103 96-97 0.3628036735044944 1103 98-99 0.3641215178902897 1103 100 0.5764598605776783 1104 1 0.23658124097173783 1104 2 0.27299483224818033 1104 3 0.23187295776869377 1104 4 0.21046946649852316 1104 5 0.18245688505118096 1104 6 0.22287790023360543 1104 7 0.2304684281096243 1104 8 0.1882041428267769 1104 9 0.22372138522212026 1104 10-11 0.28423805089356335 1104 12-13 0.29397194410947947 1104 14-15 0.31981570301394413 1104 16-17 0.32978915870131686 1104 18-19 0.3385185308628422 1104 20-21 0.3613419584854256 1104 22-23 0.410398461464645 1104 24-25 0.36169037839768237 1104 26-27 0.4151998815166209 1104 28-29 0.43392622901518507 1104 30-31 0.4289632169810744 1104 32-33 0.43231544545918865 1104 34-35 0.48600013402154474 1104 36-37 0.4243030269608994 1104 38-39 0.3616150406071412 1104 40-41 0.4258001824281763 1104 42-43 0.4666054558921928 1104 44-45 0.45756033412107655 1104 46-47 0.4745755729217649 1104 48-49 0.44178205330813114 1104 50-51 0.4578640404089427 1104 52-53 0.43455161012137467 1104 54-55 0.44219196464707267 1104 56-57 0.42556237692515 1104 58-59 0.4063116056765139 1104 60-61 0.3855518682048782 1104 62-63 0.43268273689302106 1104 64-65 0.3981343657850118 1104 66-67 0.3419030640856846 1104 68-69 0.38790921803722256 1104 70-71 0.3862600968920873 1104 72-73 0.31417673951418834 1104 74-75 0.3339905587312231 1104 76-77 0.29288597209086475 1104 78-79 0.32919500364800314 1104 80-81 0.28459824435417147 1104 82-83 0.2800387996352711 1104 84-85 0.3336155398175791 1104 86-87 0.31695660362274225 1104 88-89 0.32261466476735023 1104 90-91 0.31529033958149455 1104 92-93 0.31322377083473185 1104 94-95 0.2603786784883937 1104 96-97 0.2844885456639261 1104 98-99 0.2932191111834115 1104 100 0.6182175145693947 1105 1 0.1733671993742334 1105 2 0.14677383456356097 1105 3 0.11941114756411508 1105 4 0.17666229145106627 1105 5 0.12106875425754993 1105 6 0.14766500143366557 1105 7 0.1937648487782866 1105 8 0.15866042606182873 1105 9 0.1897408458812535 1105 10-11 0.23916100722230738 1105 12-13 0.18912528462012546 1105 14-15 0.2846365088806948 1105 16-17 0.3038326139716716 1105 18-19 0.25577969775763165 1105 20-21 0.28864816906470026 1105 22-23 0.2661315958910677 1105 24-25 0.2876395168723107 1105 26-27 0.3086516970007409 1105 28-29 0.25657093648052864 1105 30-31 0.34253470347852044 1105 32-33 0.27221289985899944 1105 34-35 0.18740511921730985 1105 36-37 0.2528668423986744 1105 38-39 0.24748025462722723 1105 40-41 0.3037095683461928 1105 42-43 0.26632914879161973 1105 44-45 0.3126265435583804 1105 46-47 0.32681695808178546 1105 48-49 0.3088501714076841 1105 50-51 0.29582516253518776 1105 52-53 0.29194049767704655 1105 54-55 0.31066106484087896 1105 56-57 0.19600317529461364 1105 58-59 0.20516827023025996 1105 60-61 0.22278245016274667 1105 62-63 0.2928098182989416 1105 64-65 0.23372202108879492 1105 66-67 0.2364176668207847 1105 68-69 0.23194486570801587 1105 70-71 0.22001191121768926 1105 72-73 0.16724170850721265 1105 74-75 0.2097806799455526 1105 76-77 0.17763171411486667 1105 78-79 0.1382242008430552 1105 80-81 0.09928521009010893 1105 82-83 0.1515164323313627 1105 84-85 0.15878359767408057 1105 86-87 0.15671586309653662 1105 88-89 0.18730052370260353 1105 90-91 0.21290760459633873 1105 92-93 0.12539198922905825 1105 94-95 0.1780010037545594 1105 96-97 0.16499612031814337 1105 98-99 0.1814383454345645 1105 100 0.3528967037375601 1106 1 0.15737526564848991 1106 2 0.22098302191322006 1106 3 0.15479436949196668 1106 4 0.12973435768097374 1106 5 0.08512053286547427 1106 6 0.1388189534646429 1106 7 0.15642646062902088 1106 8 0.07722033930226502 1106 9 0.13769960368240675 1106 10-11 0.204649431582979 1106 12-13 0.1288910975475943 1106 14-15 0.20166988064244862 1106 16-17 0.1625774547708403 1106 18-19 0.17708709559898494 1106 20-21 0.17762555264320667 1106 22-23 0.18768319436575354 1106 24-25 0.20171907295009817 1106 26-27 0.25948360774333423 1106 28-29 0.22054898978518622 1106 30-31 0.2576314211736985 1106 32-33 0.25745948841250055 1106 34-35 0.27630460209633867 1106 36-37 0.17524896135682155 1106 38-39 0.2292216119265973 1106 40-41 0.18247193749556345 1106 42-43 0.249925790738466 1106 44-45 0.21800700344316937 1106 46-47 0.2147715220115387 1106 48-49 0.24675255674133467 1106 50-51 0.2620054740640825 1106 52-53 0.2574720524024485 1106 54-55 0.20125835434363637 1106 56-57 0.20843057236000817 1106 58-59 0.17237239906894786 1106 60-61 0.12709746348337347 1106 62-63 0.16436925487910514 1106 64-65 0.17962524612048014 1106 66-67 0.22423553733177215 1106 68-69 0.17326567654778557 1106 70-71 0.15276940014180695 1106 72-73 0.13427615183082509 1106 74-75 0.1482252016779313 1106 76-77 0.16545846658038244 1106 78-79 0.1670293447094764 1106 80-81 0.16056043798175068 1106 82-83 0.16636132837139428 1106 84-85 0.15847999002091484 1106 86-87 0.0959387220974719 1106 88-89 0.1390492202769309 1106 90-91 0.12199109127386265 1106 92-93 0.159140880716798 1106 94-95 0.1305697546090414 1106 96-97 0.1293209855415327 1106 98-99 0.14298142222121157 1106 100 0.35670534865521475 1107 1 0.15614588294117482 1107 2 0.18285897790283911 1107 3 0.1730285018852129 1107 4 0.1412420135753507 1107 5 0.053534947992332604 1107 6 0.07104624192603382 1107 7 0.1407400800256653 1107 8 0.11707205642437657 1107 9 0.09267818604648426 1107 10-11 0.15872372147129 1107 12-13 0.06619275983361916 1107 14-15 0.0857012797638248 1107 16-17 0.1263562679944812 1107 18-19 0.11964684820188154 1107 20-21 0.14743672366589067 1107 22-23 0.12464823731548336 1107 24-25 0.1091466466562494 1107 26-27 0.172780342065451 1107 28-29 0.2001574719320658 1107 30-31 0.1423752939955989 1107 32-33 0.24447480297600777 1107 34-35 0.17545669263102326 1107 36-37 0.10023124108120385 1107 38-39 0.1333451606433087 1107 40-41 0.19306739286398056 1107 42-43 0.24633922637699612 1107 44-45 0.22900409064467908 1107 46-47 0.2774590104356065 1107 48-49 0.23139516489952427 1107 50-51 0.17389372915280887 1107 52-53 0.15712821870458527 1107 54-55 0.28442538970579534 1107 56-57 0.1901337998850181 1107 58-59 0.2452793220119318 1107 60-61 0.19891714051582454 1107 62-63 0.18505251041526094 1107 64-65 0.14745653272557036 1107 66-67 0.12969766921723647 1107 68-69 0.10466452044634877 1107 70-71 0.13000610542611923 1107 72-73 0.137668525002006 1107 74-75 0.11960381761653593 1107 76-77 0.17697269864280685 1107 78-79 0.10242710922855736 1107 80-81 0.015413143610132352 1107 82-83 0.1236870616522765 1107 84-85 0.08311327500415189 1107 86-87 0.02419182687428645 1107 88-89 0.06732657640765183 1107 90-91 0.01583711598721038 1107 92-93 -8.2021722579384E-4 1107 94-95 0.03100132604267003 1107 96-97 0.0539979867811482 1107 98-99 0.1137692929370715 1107 100 0.09621198343995374 1108 1 0.10606834488665129 1108 2 0.04808712630991607 1108 3 0.05734020625449432 1108 4 0.08042867883891347 1108 5 0.08493424489903134 1108 6 0.09965267405603839 1108 7 0.08434370369283783 1108 8 0.04418509365493861 1108 9 0.06526674749591166 1108 10-11 0.040102900674398256 1108 12-13 0.057275860996895744 1108 14-15 0.07705051827800702 1108 16-17 0.07142468741348296 1108 18-19 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-0.37488393618145466 2215 9 -0.49873813653637455 2215 10-11 -0.5293008123314848 2215 12-13 -0.5345644804557637 2215 14-15 -0.6445138436110227 2215 16-17 -0.678570503574818 2215 18-19 -0.6308109079160076 2215 20-21 -0.6139002062017767 2215 22-23 -0.6171745837952827 2215 24-25 -0.6388763821926347 2215 26-27 -0.663517156029215 2215 28-29 -0.5855655432459201 2215 30-31 -0.5908406025771598 2215 32-33 -0.6073830142976604 2215 34-35 -0.6199012346041641 2215 36-37 -0.7302138191037386 2215 38-39 -0.9164864380717646 2215 40-41 -0.8301838556781149 2215 42-43 -0.8421510341030327 2215 44-45 -0.900802045208323 2215 46-47 -0.8817770062891128 2215 48-49 -0.8512921444839847 2215 50-51 -0.8738635841140265 2215 52-53 -0.8349164819755259 2215 54-55 -0.7817955860656767 2215 56-57 -0.8809449614595124 2215 58-59 -0.8571885959706407 2215 60-61 -0.7737254809008505 2215 62-63 -0.8077127849684658 2215 64-65 -0.7372272923966463 2215 66-67 -0.8146469320352026 2215 68-69 -0.7953621609082688 2215 70-71 -0.808221923237852 2215 72-73 -0.8274585152300418 2215 74-75 -0.8475223099018478 2215 76-77 -0.7720014148295604 2215 78-79 -0.8215286855786275 2215 80-81 -0.8610611209354957 2215 82-83 -0.820013133702787 2215 84-85 -0.7900020736432296 2215 86-87 -0.7549432093436721 2215 88-89 -0.8068331012630949 2215 90-91 -0.7695135201055265 2215 92-93 -0.7445826952169767 2215 94-95 -0.7628800540371969 2215 96-97 -0.7071819011901752 2215 98-99 -0.7533925060334496 2215 100 -0.3845368083041478 2216 1 -0.3972101369411618 2216 2 -0.24659852500553825 2216 3 -0.2973248150446324 2216 4 -0.4111890598802006 2216 5 -0.3976552725394029 2216 6 -0.4115986690220268 2216 7 -0.41386173717742025 2216 8 -0.41342101158229383 2216 9 -0.49102603519867216 2216 10-11 -0.5377738454249368 2216 12-13 -0.5152525976265139 2216 14-15 -0.6564032672068549 2216 16-17 -0.667550639569896 2216 18-19 -0.6342960641539577 2216 20-21 -0.6434457176921882 2216 22-23 -0.6151050930940087 2216 24-25 -0.71580717811851 2216 26-27 -0.732572946723657 2216 28-29 -0.7234002444598389 2216 30-31 -0.7174177608988828 2216 32-33 -0.6351158637515013 2216 34-35 -0.6536137844679217 2216 36-37 -0.8865089608148509 2216 38-39 -0.9259544266925772 2216 40-41 -1.013482881861691 2216 42-43 -0.9580359672068468 2216 44-45 -0.9767482314427269 2216 46-47 -0.9050674722055376 2216 48-49 -0.9392469620228212 2216 50-51 -0.8759836888747401 2216 52-53 -0.8940759884702061 2216 54-55 -0.7860788034265269 2216 56-57 -0.760889878659782 2216 58-59 -0.8368152840696439 2216 60-61 -0.7673183728779307 2216 62-63 -0.8454709077230831 2216 64-65 -0.7944431171339232 2216 66-67 -0.8475742102392019 2216 68-69 -0.8165866673368711 2216 70-71 -0.8651196634264409 2216 72-73 -0.7869188632135788 2216 74-75 -0.6833749885027771 2216 76-77 -0.7677071977044037 2216 78-79 -0.7913256731575586 2216 80-81 -0.8991246764447993 2216 82-83 -0.8153023710598077 2216 84-85 -0.8434070444904052 2216 86-87 -0.8471214751860856 2216 88-89 -0.7388958447998064 2216 90-91 -0.7928208160743537 2216 92-93 -0.8947806014896216 2216 94-95 -0.8337116596228142 2216 96-97 -0.9196204112686601 2216 98-99 -0.9429202761017521 2216 100 -0.27186587829954334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 9.0 13 56.0 14 240.0 15 666.0 16 1656.0 17 3578.0 18 6405.0 19 9876.0 20 14430.0 21 20965.0 22 29514.0 23 36057.0 24 40302.0 25 45602.0 26 55508.0 27 71240.0 28 90462.0 29 114339.0 30 142461.0 31 177042.0 32 223107.0 33 288564.0 34 404220.0 35 595972.0 36 1035260.0 37 1929211.0 38 1302610.0 39 258925.0 40 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.146639742958804 20.249793252306116 12.611268673429969 25.992298331305115 2 15.107967276387877 19.637414584135495 38.469090242759655 26.78552789671697 3 18.0732636998886 25.1267629725571 28.421375355964805 28.378597971589496 4 12.540126635206631 16.209970933851224 36.942866171233476 34.30703625970867 5 13.564635386503282 38.234538165418634 34.08910591828732 14.111720529790764 6 32.272528151763964 36.974085098475 16.439844476380525 14.313542273380511 7 28.146926411984285 30.96782909794461 22.494640502335216 18.390603987735886 8 27.71719379778662 33.95900995940425 18.340959381779097 19.982836861030034 9 26.28705784804099 14.091879327477475 20.201695874685893 39.419366949795645 10-11 27.25926124573368 24.573519914822125 26.020231919814513 22.14698691962969 12-13 27.054116016351127 22.076261822433608 26.65462247541471 24.214999685800557 14-15 24.36079627310472 23.23302612933766 23.966920462789943 28.439257134767676 16-17 23.471447799197072 25.986275287181787 25.128401008624333 25.413875904996807 18-19 22.816968089593946 26.432274717032772 27.17452281669267 23.57623437668062 20-21 24.594618689121774 25.779484105614188 26.46378454462235 23.16211266064169 22-23 25.155131437898504 24.994183522371877 26.366161943783474 23.484523095946148 24-25 24.65842961627193 24.861437369401052 26.957485716108053 23.522647298218963 26-27 24.49957088525491 25.217378636195715 25.56483800220758 24.71821247634179 28-29 23.495953108680794 25.63265519123346 26.7199414945702 24.15145020551554 30-31 24.333685341969023 25.450927411083995 26.678381765324517 23.537005481622465 32-33 24.634221472623416 25.417304184022484 25.745563712717466 24.202910630636637 34-35 24.509281358787156 25.6671264281174 26.428820447328803 23.39477176576664 36-37 24.283188893825546 24.666554565083302 25.679389954780056 25.37086658631109 38-39 24.32834360488251 25.098177068799686 26.434031721365713 24.13944760495209 40-41 23.84719587794747 24.069388947315396 27.491217299980214 24.59219787475692 42-43 24.148732225315488 24.179383794024936 26.609700217655853 25.062183763003727 44-45 24.670185786630583 24.74109970203689 26.381730534369403 24.206983976963127 46-47 24.434388320367965 25.189624418624035 25.389370595513082 24.986616665494914 48-49 25.39849576103808 25.127639974168314 25.489790903971222 23.984073360822382 50-51 24.313709999485393 24.642128983018786 26.24335997437126 24.80080104312456 52-53 24.319849010763928 24.863068157521088 25.746817607583093 25.070265224131894 54-55 23.938266334517404 25.009143285392994 25.638482540782388 25.41410783930721 56-57 25.30138776445041 24.611564389675735 25.104743708963028 24.982304136910823 58-59 23.6326673145366 25.234108728501138 26.078697642271425 25.05452631469084 60-61 23.85714006150608 25.64160640602565 25.495299343843342 25.00595418862493 62-63 24.490052554865148 25.050137674757067 24.919913807411895 25.539895962965893 64-65 23.684429681493828 25.159322667295598 25.53875295073183 25.617494700478748 66-67 24.32629537618189 25.135174352445993 25.472305710605408 25.066224560766702 68-69 23.956458681989794 24.645499278466858 26.109657092370014 25.288384947173338 70-71 23.52254580157845 24.63752338468209 25.791763242900117 26.048167570839343 72-73 24.136417709473605 24.459916110608866 26.28317058149767 25.120495598419858 74-75 24.4306481695498 24.706266836588146 25.889520163172165 24.97356483068989 76-77 24.15618588278202 24.749641153769993 24.962629546315735 26.131543417132253 78-79 24.273291716580868 24.83781266199164 24.873204393300345 26.015691228127142 80-81 23.676028356868624 25.5781796200771 25.62796576939496 25.11782625365931 82-83 24.705512281284133 24.389016170894998 26.76166792660149 24.14380362121938 84-85 24.53535222486611 24.830024766235585 25.564770917756817 25.069852091141488 86-87 23.062054749543563 25.52260960889352 26.06391529758984 25.35142034397308 88-89 24.37779634862916 24.07516556123392 25.74212093779739 25.80491715233953 90-91 23.626242207550767 24.60804188733646 25.413172854118393 26.35254305099438 92-93 24.170258628499944 24.51310102696164 25.655000612451538 25.661639732086876 94-95 23.32328526253152 24.479890932890534 26.3767366987485 25.820087105829455 96-97 23.58831369985236 24.25140664535287 25.81345523414131 26.34682442065346 98-99 22.885219333754197 24.707599690077778 25.85009360723809 26.55708736892993 100 23.559437704110753 24.0069710417472 25.03403205721882 27.399559196923228 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 358.0 1 246.5 2 198.0 3 258.0 4 173.5 5 122.0 6 290.5 7 652.5 8 1041.5 9 1134.0 10 804.0 11 513.0 12 487.0 13 527.5 14 610.0 15 763.0 16 979.0 17 1273.0 18 1686.0 19 2144.0 20 2670.5 21 3382.0 22 4064.0 23 4670.5 24 5260.0 25 5958.0 26 6837.5 27 7900.5 28 9562.5 29 12861.5 30 18254.0 31 23824.0 32 32982.5 33 42989.0 34 50811.5 35 60418.0 36 72870.5 37 96115.0 38 116685.5 39 130216.0 40 135823.0 41 137110.0 42 145139.5 43 161919.5 44 190623.5 45 240847.0 46 295062.0 47 338680.0 48 372308.5 49 379074.0 50 369355.0 51 389687.0 52 406387.0 53 394757.5 54 386331.0 55 337588.0 56 292954.0 57 247744.5 58 198243.5 59 195964.5 60 164963.5 61 113146.5 62 84679.5 63 70618.0 64 51672.0 65 25334.5 66 14310.0 67 10109.5 68 7481.5 69 5503.5 70 4023.5 71 2942.5 72 2074.5 73 1493.0 74 949.5 75 623.0 76 373.0 77 124.5 78 42.0 79 13.5 80 7.5 81 3.0 82 1.0 83 0.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007088492361750844 3 0.0 4 0.08626506757623571 5 0.004493727264095627 6 4.348768320092542E-5 7 5.7983577601233895E-5 8 0.0 9 0.0 10-11 0.0 12-13 2.971658352063237E-4 14-15 0.009357099835399119 16-17 0.0 18-19 4.348768320092542E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.247947200154237E-6 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.4495894400308474E-5 60-61 0.0 62-63 0.0 64-65 6.523152480138814E-5 66-67 1.0147126080215929E-4 68-69 0.0 70-71 3.6964530720786607E-4 72-73 2.391822576050898E-4 74-75 3.551494128075576E-4 76-77 1.0147126080215929E-4 78-79 1.4495894400308474E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.061897469089317175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6898505.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.735690698887396 #Duplication Level Percentage of deduplicated Percentage of total 1 68.55611392276451 38.89578475039655 2 16.796274479084353 19.058964674778917 3 6.563692340823795 11.17186855474905 4 3.1380781389890164 7.121641227304841 5 1.6734806273405167 4.74730396316858 6 1.0036419327349535 3.4165390960850326 7 0.6383285525730434 2.5351207926136876 8 0.4114513554621971 1.8675181472912952 9 0.2704807889893295 1.3811322945680662 >10 0.9270132349814071 8.008163232098386 >50 0.014739759004350577 0.5519350423763151 >100 0.006065800293543805 0.5995077568251732 >500 4.6864912543335453E-4 0.18892786068448905 >1k 8.520891678034119E-5 0.13797339776613465 >5k 8.520891678034119E-5 0.31761920929344944 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7082 0.1026599241429846 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8991788800616944E-5 2 0.0 0.0 0.0 0.0 2.8991788800616944E-5 3 0.0 0.0 0.0 0.0 2.8991788800616944E-5 4 0.0 0.0 0.0 0.0 2.8991788800616944E-5 5 0.0 0.0 0.0 0.0 2.8991788800616944E-5 6 0.0 0.0 0.0 0.0 2.8991788800616944E-5 7 0.0 0.0 0.0 0.0 2.8991788800616944E-5 8 0.0 0.0 0.0 0.0 2.8991788800616944E-5 9 0.0 0.0 0.0 0.0 2.8991788800616944E-5 10-11 0.0 0.0 0.0 0.0 2.8991788800616944E-5 12-13 0.0 0.0 0.0 1.4495894400308472E-5 1.3046304960277625E-4 14-15 0.0 0.0 0.0 2.174384160046271E-5 1.5220689120323897E-4 16-17 0.0 0.0 0.0 3.623973600077118E-5 1.811986800038559E-4 18-19 0.0 0.0 0.0 5.073563040107965E-5 2.029425216043186E-4 20-21 0.0 0.0 0.0 6.523152480138812E-5 2.174384160046271E-4 22-23 0.0 0.0 0.0 1.6670278560354743E-4 2.174384160046271E-4 24-25 0.0 0.0 0.0 3.6964530720786607E-4 2.174384160046271E-4 26-27 0.0 0.0 0.0 7.102988256151152E-4 2.5367815200539825E-4 28-29 0.0 0.0 0.0 0.0030441378240647792 2.609260992055525E-4 30-31 0.0 0.0 0.0 0.012379493817863437 2.609260992055525E-4 32-33 0.0 0.0 0.0 0.02997026167263777 2.609260992055525E-4 34-35 0.0 0.0 0.0 0.05531633303157713 2.609260992055525E-4 36-37 0.0 0.0 0.0 0.09968101784372121 2.609260992055525E-4 38-39 0.0 0.0 0.0 0.1665288348707437 2.6817404640570674E-4 40-41 0.0 0.0 0.0 0.23994329206110598 2.75421993605861E-4 42-43 0.0 0.0 0.0 0.31787322035716437 2.826699408060152E-4 44-45 0.0 0.0 0.0 0.41283582457358514 3.0441378240647795E-4 46-47 0.0 0.0 0.0 0.5323254821153279 3.261576240069406E-4 48-49 0.0 0.0 0.0 0.6745374541295541 3.406535184072491E-4 50-51 0.0 0.0 0.0 0.8248888708495536 3.4790146560740334E-4 52-53 0.0 0.0 0.0 0.97133364402867 4.348768320092542E-4 54-55 0.0 0.0 0.0 1.1375580651170072 4.493727264095627E-4 56-57 0.0 0.0 0.0 1.34445071794541 4.493727264095627E-4 58-59 0.0 0.0 0.0 1.5917506764146725 4.493727264095627E-4 60-61 0.0 0.0 0.0 1.8552642927706802 4.8561246241033383E-4 62-63 0.0 0.0 0.0 2.1278233472324803 5.653398816120304E-4 64-65 0.0 0.0 0.0 2.415791537441808 5.798357760123389E-4 66-67 0.0 0.0 0.0 2.742992865845571 5.798357760123389E-4 68-69 0.0 0.0 0.0 3.1109783931446016 5.798357760123389E-4 70-71 0.0 0.0 0.0 3.526967074750254 5.870837232124931E-4 72-73 0.0 0.0 0.0 3.962271535644317 6.378193536135729E-4 74-75 0.0 0.0 0.0 4.3882623843861825 6.958029312148067E-4 76-77 0.0 0.0 0.0 4.874085037265321 7.102988256151152E-4 78-79 0.0 0.0 0.0 5.400836847983729 7.247947200154237E-4 80-81 0.0 0.0 0.0 5.963589212445305 7.247947200154237E-4 82-83 0.0 0.0 0.0 6.563211884314065 7.247947200154237E-4 84-85 0.0 0.0 0.0 7.145395995219253 7.537865088160406E-4 86-87 0.0 0.0 0.0 7.75517304111543 7.682824032163491E-4 88 0.0 0.0 0.0 8.248961187967538 7.97274192016966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 10315 0.0 42.85187 94 GTACTAG 10480 0.0 35.779305 1 CAGTACG 1390 0.0 33.817543 9 TACCCCG 530 0.0 33.698536 5 GTATCAA 18590 0.0 33.659454 1 TAGTACT 14355 0.0 32.804085 4 CTGTGCG 14505 0.0 32.504242 9 CAACGCA 17820 0.0 31.597483 5 TAGGCAT 13285 0.0 31.345524 5 GGTATCA 12025 0.0 31.299667 1 ACTGTGC 15925 0.0 30.928806 8 GTACTGT 15680 0.0 30.482452 6 ATCAACG 18775 0.0 29.967344 3 TACTGTG 16935 0.0 29.805555 7 CTAACGC 4820 0.0 29.18241 3 TCTAACG 4935 0.0 28.886194 2 CTAGGCA 14865 0.0 28.830389 4 TAACGCC 4895 0.0 28.735285 4 CTAGTAC 17090 0.0 28.682875 3 CGCCTAC 4950 0.0 28.673447 7 >>END_MODULE