FastQCFastQC Report
Mon 16 Jan 2017
ERR1146686_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146686_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4383607
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60150.032.8287661
GTACTAG39750.032.053321
GTATCAA90000.031.2914141
GGGCACC47750.030.00599394
CAACGCA86750.028.9260855
TAGTACT53650.028.9065844
CTGTGCG56350.028.3526089
ATCAACG89100.028.1654153
TAGGCAT48200.027.9803265
CTAGTAC61900.025.8890613
GTACTGT61450.025.6174496
TATCAAC109750.025.5213832
GCATCGT44100.025.2532798
ACTGTGC67550.024.8342388
CTAGGCA56700.024.5335984
AACGCAG108750.024.4136166
GTACATG181050.024.2543261
TCTAACG19200.024.23242
CTAACGC19150.024.0497113
TACATGG182100.023.7948762
Produced by FastQC (version 0.11.5)